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Article

Effect of the hsa‑microRNA 200c‑3p/ZEB1 loop in epithelial‑mesenchymal transition of ovarian cancer cells

  • Authors:
    • Merve Senturk
    • Ece Gumusoglu‑Acar
    • Samet Topuz
    • Tuba Gunel
  • View Affiliations / Copyright

    Affiliations: Molecular Biology and Genetics Programme, Institute of Graduate Studies in Sciences, Istanbul University, Istanbul 34134, Turkey, Department of Molecular Biology and Genetics, Faculty of Science, Istanbul University, Istanbul 34134, Turkey, Department of Obstetrics and Gynecology, Istanbul Medical Faculty, Istanbul University, Istanbul 34104, Turkey
  • Article Number: 131
    |
    Published online on: February 10, 2026
       https://doi.org/10.3892/ol.2026.15484
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Abstract

Ovarian cancer (OC) is a disease often diagnosed at advanced stages with distant metastasis, which is characterized by the epithelial‑mesenchymal transition (EMT) of cells. The aim of the present study was to analyze the target genes of Homo sapiens microRNA 200c‑3p (hsa‑miR‑200c‑3p) and their associated proteins, which have a role in the EMT process. The expression level of hsa‑miR‑200c‑3p was analyzed in ovarian tissues obtained from healthy individuals (n=15) and patients with advanced epithelial OC (EOC; n=15). Zinc finger enhancer‑box binding homeobox 1 (ZEB1) is one of the target genes of hsa‑miR‑200c‑3p and was identified using in silico methods. The expression level of ZEB1 was analyzed in both groups. ZEB1, vimentin and E‑cadherin proteins were analyzed using immunohistochemistry (IHC) in healthy and OC tissues. The expression of hsa‑miR‑200c‑3p was significantly increased in patients with OC (P<0.01). ZEB1 expression decreased significantly in the EOC group (P<0.01). The correlation analysis indicated a strong negative correlation between hsa‑miR‑200c‑3p and the ZEB1 gene (ρ=‑0.75; P<0.01). IHC staining revealed that the higher staining intensity of ZEB1 and E‑cadherin proteins were found in cancerous tissues compared with healthy ones (P<0.05). However, the intensity and extent of vimentin staining was decreased in cancerous tissues (P<0.05). The decreased expression of ZEB1 is regulated by hsa‑miR‑200c‑3p. The downregulation of ZEB1 may cause insufficient binding to the mRNA of E‑cadherin, leading to increased E‑cadherin expression. The hsa‑miR‑200c‑3p/ZEB1 loop regulates vimentin and E‑cadherin expression in OC. The increased expression level of hsa‑miR‑200c‑3p may also cause mesenchymal‑to‑epithelial transition in OC cells.
View Figures

Figure 1

Expression and receiver operating
characteristic curve analysis of hsa-miR-200c-3p. (A) Relative
expression of hsa-miR-200c-3p in healthy and cancerous ovarian
tissues. (B) Receiver operating characteristic curve analysis of
hsa-miR-200c-3p in predicting ovarian cancer. **P<0.01.
hsa-miR-200c-3p, Homo sapiens microRNA-200c-3p; AUC, area
under the curve.

Figure 2

The expression and receiver operating
characteristic curve analyses of ZEB1. (A) Relative expression of
the ZEB1 gene in healthy and cancerous ovarian tissues. (B)
Receiver operating characteristic curve analysis of the ZEB1 gene
mRNAs in predicting ovarian cancer. ***P<0.001. ZEB1, zinc
finger enhancer-box homeobox 1; AUC, area under the curve.

Figure 3

Semi-quantitative scoring results of
immunohistochemical staining of ZEB1, E-Cad and Vim antibodies.
***P<0.001. HOT, healthy ovarian tissue; OCT, ovarian cancer
tissue; E-Cad, E-cadherin; Vim, vimentin; ZEB1, zinc finger
enhancer-box homeobox 1.

Figure 4

IHC staining samples of ZEB1 protein
from (A) healthy ovarian tissues and (B) cancerous ovarian tissues.
A sample of IHC staining of E-cadherin of (C) healthy ovarian
tissues and (D) cancerous ovarian tissues. A sample of IHC staining
of vimentin from (E) healthy ovarian tissues and (F) cancerous
ovarian tissues. Magnification, ×20. IHC, immunohistochemical;
ZEB1, zinc finger enhancer-box homeobox 1.
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Copy and paste a formatted citation
Spandidos Publications style
Senturk M, Gumusoglu‑Acar E, Topuz S and Gunel T: Effect of the hsa‑microRNA 200c‑3p/ZEB1 loop in epithelial‑mesenchymal transition of ovarian cancer cells. Oncol Lett 31: 131, 2026.
APA
Senturk, M., Gumusoglu‑Acar, E., Topuz, S., & Gunel, T. (2026). Effect of the hsa‑microRNA 200c‑3p/ZEB1 loop in epithelial‑mesenchymal transition of ovarian cancer cells. Oncology Letters, 31, 131. https://doi.org/10.3892/ol.2026.15484
MLA
Senturk, M., Gumusoglu‑Acar, E., Topuz, S., Gunel, T."Effect of the hsa‑microRNA 200c‑3p/ZEB1 loop in epithelial‑mesenchymal transition of ovarian cancer cells". Oncology Letters 31.4 (2026): 131.
Chicago
Senturk, M., Gumusoglu‑Acar, E., Topuz, S., Gunel, T."Effect of the hsa‑microRNA 200c‑3p/ZEB1 loop in epithelial‑mesenchymal transition of ovarian cancer cells". Oncology Letters 31, no. 4 (2026): 131. https://doi.org/10.3892/ol.2026.15484
Copy and paste a formatted citation
x
Spandidos Publications style
Senturk M, Gumusoglu‑Acar E, Topuz S and Gunel T: Effect of the hsa‑microRNA 200c‑3p/ZEB1 loop in epithelial‑mesenchymal transition of ovarian cancer cells. Oncol Lett 31: 131, 2026.
APA
Senturk, M., Gumusoglu‑Acar, E., Topuz, S., & Gunel, T. (2026). Effect of the hsa‑microRNA 200c‑3p/ZEB1 loop in epithelial‑mesenchymal transition of ovarian cancer cells. Oncology Letters, 31, 131. https://doi.org/10.3892/ol.2026.15484
MLA
Senturk, M., Gumusoglu‑Acar, E., Topuz, S., Gunel, T."Effect of the hsa‑microRNA 200c‑3p/ZEB1 loop in epithelial‑mesenchymal transition of ovarian cancer cells". Oncology Letters 31.4 (2026): 131.
Chicago
Senturk, M., Gumusoglu‑Acar, E., Topuz, S., Gunel, T."Effect of the hsa‑microRNA 200c‑3p/ZEB1 loop in epithelial‑mesenchymal transition of ovarian cancer cells". Oncology Letters 31, no. 4 (2026): 131. https://doi.org/10.3892/ol.2026.15484
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