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Article Open Access

FAM122B acts as an independent poor prognostic factor and promotes the malignant biological behaviors of low‑grade glioma

  • Authors:
    • Hao Wang
    • Tengfei Guo
    • Yanbo Gao
    • Yi Zhou
    • Rongjun Qian
  • View Affiliations / Copyright

    Affiliations: The First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan 450003, P.R. China, Department of Neurosurgery, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 427
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    Published online on: July 28, 2026
       https://doi.org/10.3892/ol.2026.15782
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Abstract

The aim of the present study was to investigate the role and mechanism of Family with sequence similarity 122, member B (FAM122B) in low‑grade glioma (LGG) by combining bioinformatics analysis of public databases and in vitro cell experiments. Data from databases including Gene Expression Omnibus, The Cancer Genome Atlas and Chinese Glioma Genome Atlas were integrated to analyze the expression characteristics, clinical correlations and molecular mechanisms of FAM122B. Meanwhile, SHG44 cells were used as the research object and short interfering RNA was applied to knockdown FAM122B expression to verify its effects on cell functions. The results showed that FAM122B was markedly highly expressed in LGG tissues and cell lines compared with normal brain tissues and normal glial cells, which was closely associated with the malignant clinical features of LGG. Patients with high FAM122B expression had a poorer prognosis, and FAM122B was identified as an independent adverse prognostic marker for LGG. In vitro experiments confirmed that knockdown of FAM122B could markedly inhibit the proliferation and migration of SHG44 and SW1088 cells. In conclusion, FAM122B plays a key oncogenic role in LGG and it was hypothesized to serve as a potential biomarker for prognostic evaluation and a novel target for the targeted therapy of LGG, providing a theoretical basis for its precise diagnosis and treatment.
View Figures

Figure 1

FAM122B is highly expressed in
LGG tissues and cell lines. (A) mRNA expression levels of
FAM122B in normal brain tissues (n=9) and LGG tumor tissues
(n=12) from the GSE35493 dataset. (B) RT-qPCR results showing the
relative mRNA expression of FAM122B in the human normal
astrocyte cell line HA1800 and LGG cell lines SW1088 and SHG44. (C)
Quantification of the IHC staining results in panels (D) and (E),
showing the average optical density of FAM122B protein
expression in normal cerebral cortex tissues (n=3) and LGG tumor
tissues (n=5). Representative IHC staining image of FAM122B
in (D) normal cerebral cortex tissues and (E) in LGG tumor tissues
from the HPA database. All original magnification, ×4.3.
*P<0.05, **P<0.01. FAM122B, Family with sequence
similarity 122, member B; LGG, low-grade glioma; RT-qPCR, reverse
transcription-quantitative PCR; IHC, immunohistochemistry; HPA,
Human Protein Atlas.

Figure 2

Correlation analysis between
FAM122B expression level and clinicopathological
characteristics of patients with LGG. (A) Comparison of
FAM122B mRNA expression levels in LGG tissues with different
WHO grades (grade II vs. grade III) based on TCGA RNA-seq dataset.
(B) Comparison of FAM122B mRNA expression levels in LGG
tissues with different WHO grades (grade II vs. grade III) based on
CGGA RNA-seq dataset. (C) Comparison of FAM122B mRNA
expression levels in LGG tissues with different WHO grades (grade
II vs. grade III) based on CGGA microarray dataset. (D) Comparison
of FAM122B mRNA expression levels between primary and
recurrent LGG tissues in the TCGA RNA-seq dataset. (E) Comparison
of FAM122B mRNA expression levels between primary and
recurrent LGG tissues in the CGGA RNA-seq dataset. (F) Comparison
of FAM122B mRNA expression levels between primary and
recurrent LGG tissues in the CGGA microarray dataset. (G)
Comparison of FAM122B mRNA expression levels in LGG tissues
with different IDH mutation statuses based on TCGA RNA-seq dataset.
(H) Comparison of FAM122B mRNA expression levels in LGG
tissues with different IDH mutation statuses based on CGGA RNA-seq
dataset. (I) Comparison of FAM122B mRNA expression levels in
LGG tissues with different 1p19q co-deletion statuses based on the
CGGA RNA-seq dataset. FAM122B, Family with sequence
similarity 122, member B; LGG, low-grade glioma; WHO, World Health
Organization; TCGA, The Cancer Genome Atlas; CGGA, Chinese Glioma
Genome Atlas.

Figure 3

Prognostic value of FAM122B
expression level in patients with LGG. (A) Kaplan-Meier survival
curves of patients with LGG stratified by FAM122B expression
levels based on TCGA RNA-seq dataset. (B) Kaplan-Meier survival
curves of patients with LGG stratified by FAM122B expression
levels based on CGGA RNA-seq dataset. (C) Kaplan-Meier survival
curves of patients with LGG stratified by FAM122B expression
levels based on CGGA microarray dataset. (D) ROC curves and AUC
values of FAM122B for predicting 1-, 3- and 5-year survival
outcomes of patients with LGG based on the TCGA RNA-seq dataset.
(E) ROC curves and AUC values of FAM122B for predicting 1-,
3- and 5-year survival outcomes of patients with LGG based on the
CGGA RNA-seq dataset. (F) ROC curves and AUC values of
FAM122B for predicting 1-, 3- and 5-year survival outcomes
of patients with LGG based on the CGGA microarray dataset.
FAM122B, Family with sequence similarity 122, member B; LGG,
low-grade glioma; TCGA, The Cancer Genome Atlas; CGGA, Chinese
Glioma Genome Atlas; ROC, plot receiver operating characteristic;
AUC, area under the curve.

Figure 4

Validation of FAM122B as an
independent poor prognostic factor in patients with LGG. (A) Forest
plots of univariate Cox proportional hazards regression analysis
based on TCGA RNA-seq dataset. (B) Forest plots of multivariate Cox
proportional hazards regression analysis based on the TCGA RNA-seq
dataset. (C) Forest plots of univariate Cox proportional hazards
regression analysis based on CGGA RNA-seq dataset. (D) Forest plots
of multivariate Cox proportional hazards regression analysis based
on the CGGA RNA-seq dataset. (E) Forest plots of univariate Cox
proportional hazards regression analysis based on CGGA microarray
dataset. (F) Forest plots of multivariate Cox proportional hazards
regression analysis based on the CGGA microarray dataset. (G)
Forest plot of meta-analysis across multiple datasets,
comprehensively evaluating the prognostic HR and 95% CI of
FAM122B in patients with LGG. FAM122B, Family with
sequence similarity 122, member B; LGG, low-grade glioma; TCGA, The
Cancer Genome Atlas; CGGA, Chinese Glioma Genome Atlas; HR, hazard
ratio; CI, confidence interval.

Figure 5

Co-expression gene analysis and GSEA
of FAM122B. (A) Chord diagram of FAM122B co-expressed
genes, where red indicates positive correlation and green indicates
negative correlation; color intensity reflects the magnitude of the
correlation coefficient. (B) The top five positively and top five
negatively correlated genes with FAM122B, along with their
correlation coefficients and P-values. (C) GSEA enrichment plots
showing the enrichment of extracellular matrix receptor interaction
pathway in FAM122B high-expression samples. (D) GSEA
enrichment plots showing the enrichment of Toll-like receptor
signaling pathway in FAM122B high-expression samples. (E)
GSEA enrichment plots showing the enrichment of focal adhesion
pathway in FAM122B high-expression samples. (F) Statistical
results of FDR q-values and P-values for each enriched pathway.
GSEA, gene set enrichment analysis; FAM122B, Family with
sequence similarity 122, member B; FDR, false discovery rate.

Figure 6

FAM122B affects LGG immune
microenvironment and malignant cell behaviors. (A) TME scores of
patients with LGG in FAM122B high- and low-expression
groups, calculated by the ESTIMATE algorithm. (B) Correlation
analysis between FAM122B expression level and infiltration
levels. (C) Colony formation assay to detect the proliferation
capacity of SHG44 and SW1088 cells after FAM122B knockdown.
(D) CCK-8 assay to detect the proliferation viability of SHG44 and
SW1088 cells after FAM122B knockdown. (E) Transwell assay to
detect the invasion capacity of SHG44 and SW1088 cells after
FAM122B knockdown. Scale bar, 100 µm. (F) Wound healing
assay to detect the migration capacity of SHG44 and SW1088 cells
after FAM122B knockdown. Scale bar, 100 µm. *P<0.05,
**P<0.01, ***P<0.001, ****P<0.0001. FAM122B, Family
with sequence similarity 122, member B; LGG, low-grade glioma; TME,
tumor microenvironment; ns, no significance; si, short interfering;
LGG, low-grade glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Wang H, Guo T, Gao Y, Zhou Y and Qian R: <em>FAM122B</em> acts as an independent poor prognostic factor and promotes the malignant biological behaviors of low‑grade glioma. Oncol Lett 32: 427, 2026.
APA
Wang, H., Guo, T., Gao, Y., Zhou, Y., & Qian, R. (2026). <em>FAM122B</em> acts as an independent poor prognostic factor and promotes the malignant biological behaviors of low‑grade glioma. Oncology Letters, 32, 427. https://doi.org/10.3892/ol.2026.15782
MLA
Wang, H., Guo, T., Gao, Y., Zhou, Y., Qian, R."<em>FAM122B</em> acts as an independent poor prognostic factor and promotes the malignant biological behaviors of low‑grade glioma". Oncology Letters 32.4 (2026): 427.
Chicago
Wang, H., Guo, T., Gao, Y., Zhou, Y., Qian, R."<em>FAM122B</em> acts as an independent poor prognostic factor and promotes the malignant biological behaviors of low‑grade glioma". Oncology Letters 32, no. 4 (2026): 427. https://doi.org/10.3892/ol.2026.15782
Copy and paste a formatted citation
x
Spandidos Publications style
Wang H, Guo T, Gao Y, Zhou Y and Qian R: <em>FAM122B</em> acts as an independent poor prognostic factor and promotes the malignant biological behaviors of low‑grade glioma. Oncol Lett 32: 427, 2026.
APA
Wang, H., Guo, T., Gao, Y., Zhou, Y., & Qian, R. (2026). <em>FAM122B</em> acts as an independent poor prognostic factor and promotes the malignant biological behaviors of low‑grade glioma. Oncology Letters, 32, 427. https://doi.org/10.3892/ol.2026.15782
MLA
Wang, H., Guo, T., Gao, Y., Zhou, Y., Qian, R."<em>FAM122B</em> acts as an independent poor prognostic factor and promotes the malignant biological behaviors of low‑grade glioma". Oncology Letters 32.4 (2026): 427.
Chicago
Wang, H., Guo, T., Gao, Y., Zhou, Y., Qian, R."<em>FAM122B</em> acts as an independent poor prognostic factor and promotes the malignant biological behaviors of low‑grade glioma". Oncology Letters 32, no. 4 (2026): 427. https://doi.org/10.3892/ol.2026.15782
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