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Article

Chemoprevention of 1,2-dimethylhydrazine-induced colonic preneoplastic lesions in Fischer rats by 6-methylsulfinylhexyl isothiocyanate, a wasabi derivative

  • Authors:
    • Toshiya Kuno
    • Yoshinobu Hirose
    • Yasuhiro Yamada
    • Katsumi Imaida
    • Kenjiro Tatematsu
    • Yukio Mori
    • Hideki Mori
  • View Affiliations / Copyright

    Affiliations: Onco-Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan
  • Pages: 273-278
    |
    Published online on: March 1, 2010
       https://doi.org/10.3892/ol_00000048
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Abstract

The preventive effects of dietary exposure to a wasabi derivative 6-methylsulfinylhexyl isothiocyanate (6-MSITC) during the initiation and post-initiation phases on the development of 1,2-dimethylhydrazine (DMH)-induced colonic aberrant crypt foci (ACF), and β-catenin-accumulated crypts (BCAC) were investigated in male F344 rats. To induce ACF and BCAC, rats were given four weekly subcutaneous injections of DMH (40 mg/kg body weight). The rats also received diets containing 200 or 400 ppm 6-MSITC during the initiation or post-initiation phases. The experiment was terminated 12 weeks after the start. DMH exposure produced a substantial number of ACF (323.8±69.7/colon) and BCAC (3.80±1.05/cm2 at the end of the study. Dietary administration of 6-MSITC at a dose of 400 ppm during the initiation phase caused a significant reduction in the total number of ACF (52% reduction, P<0.0001), larger ACF (4 or more crypt ACF) (58% reduction, P<0.001) and BCAC (76% reduction, P<0.00001). The dietary exposure to 6-MSITC significantly reduced the size (crypt multiplicity) of BCAC during both initiation and post-initiation treatment when compared to group 1 treated with DMH alone. Immunohistochemically, 6-MSITC administration lowered the proliferating cell nuclear antigen labeling index in ACF and BCAC. In addition, protein levels of hepatic cytochrome P-450 isozymes at 24 h after 6-MSITC exposure were significantly suppressed (P<0.01). The results indicated that 6-MSITC exerted chemopreventive effects in the present short-term colon carcinogenesis bioassay, through alterations in cell proliferation activity and drug metabolizing enzyme levels.
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Copy and paste a formatted citation
Spandidos Publications style
Kuno T, Hirose Y, Yamada Y, Imaida K, Tatematsu K, Mori Y and Mori H: Chemoprevention of 1,2-dimethylhydrazine-induced colonic preneoplastic lesions in Fischer rats by 6-methylsulfinylhexyl isothiocyanate, a wasabi derivative . Oncol Lett 1: 273-278, 2010.
APA
Kuno, T., Hirose, Y., Yamada, Y., Imaida, K., Tatematsu, K., Mori, Y., & Mori, H. (2010). Chemoprevention of 1,2-dimethylhydrazine-induced colonic preneoplastic lesions in Fischer rats by 6-methylsulfinylhexyl isothiocyanate, a wasabi derivative . Oncology Letters, 1, 273-278. https://doi.org/10.3892/ol_00000048
MLA
Kuno, T., Hirose, Y., Yamada, Y., Imaida, K., Tatematsu, K., Mori, Y., Mori, H."Chemoprevention of 1,2-dimethylhydrazine-induced colonic preneoplastic lesions in Fischer rats by 6-methylsulfinylhexyl isothiocyanate, a wasabi derivative ". Oncology Letters 1.2 (2010): 273-278.
Chicago
Kuno, T., Hirose, Y., Yamada, Y., Imaida, K., Tatematsu, K., Mori, Y., Mori, H."Chemoprevention of 1,2-dimethylhydrazine-induced colonic preneoplastic lesions in Fischer rats by 6-methylsulfinylhexyl isothiocyanate, a wasabi derivative ". Oncology Letters 1, no. 2 (2010): 273-278. https://doi.org/10.3892/ol_00000048
Copy and paste a formatted citation
x
Spandidos Publications style
Kuno T, Hirose Y, Yamada Y, Imaida K, Tatematsu K, Mori Y and Mori H: Chemoprevention of 1,2-dimethylhydrazine-induced colonic preneoplastic lesions in Fischer rats by 6-methylsulfinylhexyl isothiocyanate, a wasabi derivative . Oncol Lett 1: 273-278, 2010.
APA
Kuno, T., Hirose, Y., Yamada, Y., Imaida, K., Tatematsu, K., Mori, Y., & Mori, H. (2010). Chemoprevention of 1,2-dimethylhydrazine-induced colonic preneoplastic lesions in Fischer rats by 6-methylsulfinylhexyl isothiocyanate, a wasabi derivative . Oncology Letters, 1, 273-278. https://doi.org/10.3892/ol_00000048
MLA
Kuno, T., Hirose, Y., Yamada, Y., Imaida, K., Tatematsu, K., Mori, Y., Mori, H."Chemoprevention of 1,2-dimethylhydrazine-induced colonic preneoplastic lesions in Fischer rats by 6-methylsulfinylhexyl isothiocyanate, a wasabi derivative ". Oncology Letters 1.2 (2010): 273-278.
Chicago
Kuno, T., Hirose, Y., Yamada, Y., Imaida, K., Tatematsu, K., Mori, Y., Mori, H."Chemoprevention of 1,2-dimethylhydrazine-induced colonic preneoplastic lesions in Fischer rats by 6-methylsulfinylhexyl isothiocyanate, a wasabi derivative ". Oncology Letters 1, no. 2 (2010): 273-278. https://doi.org/10.3892/ol_00000048
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