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March-April 2010 Volume 1 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

Abundance of a distinct cluster of telomere t-stumps in advanced breast cancer cell line

  • Authors:
    • Rekha B. Pai
    • S. Balakrishna Pai
    • Lily Yang
    • Harish C. Joshi
  • View Affiliations / Copyright

    Affiliations: Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA
  • Pages: 339-343
    |
    Published online on: March 1, 2010
       https://doi.org/10.3892/ol_00000060
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Abstract

Breast tumors are the second major cause of cancer-related death in women worldwide. These tumors are aggressive, leading to metastatic cancers that are heterogeneous in nature, with numerous subtypes. The basal-like tumor subtype invariably shows unfavorable prognosis and is often characterized by the lack of estrogen, progesterone and HER2 receptors. These cancer types do not respond to the current targeted therapies. Therefore, the need for the discovery of novel diagnostic markers/therapeutic targets is of paramount importance. Immortalization of breast tumor cells leading to advanced stage cancer is one of the pivotal steps in breast cancer and telomeres/telomerase play a critical role in this process. Using single telomere length analysis, cell lines with a basal-like phenotype encompassing immortalized/non-tumorigenic MCF10A and invasive/metastatic MCF10CA1 along with the MCF-7 cell line were examined for the presence of a unique class of telomere t-stumps. Telomerase activity, protein levels of telomerase and bulk telomere lengths were assessed in the above-mentioned cell lines. This is the first study describing the existence of a distinct class of extremely short telomeres termed ‘t-stumps’ in breast cancer cell lines. The cell lines MCF10A and MCF10CA1 showed distinct telomeric bands in the molecular size range of 100-1,000 bp, whereas the MCF-7 cell line showed very low levels of t-stumps. Of note is that only the highly invasive/metastatic cancer cell line MCF10CA1 exhibited an abundance of a cluster of t-stumps with a size distribution range of 500-700 bp. These unique t-stumps observed in the advanced breast cancer cell line may serve as a novel diagnostic marker and also form a key molecular target for novel anticancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Pai RB, Balakrishna Pai S, Yang L and Joshi HC: Abundance of a distinct cluster of telomere t-stumps in advanced breast cancer cell line . Oncol Lett 1: 339-343, 2010.
APA
Pai, R.B., Balakrishna Pai, S., Yang, L., & Joshi, H.C. (2010). Abundance of a distinct cluster of telomere t-stumps in advanced breast cancer cell line . Oncology Letters, 1, 339-343. https://doi.org/10.3892/ol_00000060
MLA
Pai, R. B., Balakrishna Pai, S., Yang, L., Joshi, H. C."Abundance of a distinct cluster of telomere t-stumps in advanced breast cancer cell line ". Oncology Letters 1.2 (2010): 339-343.
Chicago
Pai, R. B., Balakrishna Pai, S., Yang, L., Joshi, H. C."Abundance of a distinct cluster of telomere t-stumps in advanced breast cancer cell line ". Oncology Letters 1, no. 2 (2010): 339-343. https://doi.org/10.3892/ol_00000060
Copy and paste a formatted citation
x
Spandidos Publications style
Pai RB, Balakrishna Pai S, Yang L and Joshi HC: Abundance of a distinct cluster of telomere t-stumps in advanced breast cancer cell line . Oncol Lett 1: 339-343, 2010.
APA
Pai, R.B., Balakrishna Pai, S., Yang, L., & Joshi, H.C. (2010). Abundance of a distinct cluster of telomere t-stumps in advanced breast cancer cell line . Oncology Letters, 1, 339-343. https://doi.org/10.3892/ol_00000060
MLA
Pai, R. B., Balakrishna Pai, S., Yang, L., Joshi, H. C."Abundance of a distinct cluster of telomere t-stumps in advanced breast cancer cell line ". Oncology Letters 1.2 (2010): 339-343.
Chicago
Pai, R. B., Balakrishna Pai, S., Yang, L., Joshi, H. C."Abundance of a distinct cluster of telomere t-stumps in advanced breast cancer cell line ". Oncology Letters 1, no. 2 (2010): 339-343. https://doi.org/10.3892/ol_00000060
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