Human mast cells induce caspase-independent DNA fragmentation in leukemic T cells

  • Authors:
    • Sandra J. Gallagher
    • Jean S. Marshall
    • David W. Hoskin
  • View Affiliations

  • Published online on: July 1, 2003     https://doi.org/10.3892/or.10.4.1019
  • Pages: 1019-1023
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Abstract

Mast cells are known to play an important role in inflammation and host defense. Recent evidence suggests that mast cells may also participate in immune surveillance against cancer cells. In this study, we demonstrate that human mast cells are able to trigger apoptosis in Jurkat T leukemia cells. Fragmentation of Jurkat cell DNA was detectable by JAM assay within 4 h of exposure to HMC-1 mast cells or cultured human cord blood-derived mast cells. HMC-1 mast cells that were fixed with paraformaldehyde retained the ability to induce apoptosis in Jurkat cells while cell-free conditioned supernatants from HMC-1 cell cultures failed to elicit DNA fragmentation, suggesting that mast cells mediate anti-tumor activity via a cell-surface molecule. Surprisingly, the apoptosis-inducing activity of HMC-1 mast cells was not mediated by known tumor necrosis factor (TNF) superfamily members (TNF-α, Fas ligand, TRAIL, TWEAK), nor did the fragmentation of Jurkat cell DNA by mast cells require the activity of caspase-3, -8, or -9. Collectively, these data indicate that mast cells trigger caspase-independent apoptosis in leukemic cells via a novel cell-surface molecule, and are consistent with a role for mast cells as anti-tumor effector cells.

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July-August 2003
Volume 10 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Gallagher SJ, Marshall JS and Hoskin DW: Human mast cells induce caspase-independent DNA fragmentation in leukemic T cells. Oncol Rep 10: 1019-1023, 2003
APA
Gallagher, S.J., Marshall, J.S., & Hoskin, D.W. (2003). Human mast cells induce caspase-independent DNA fragmentation in leukemic T cells. Oncology Reports, 10, 1019-1023. https://doi.org/10.3892/or.10.4.1019
MLA
Gallagher, S. J., Marshall, J. S., Hoskin, D. W."Human mast cells induce caspase-independent DNA fragmentation in leukemic T cells". Oncology Reports 10.4 (2003): 1019-1023.
Chicago
Gallagher, S. J., Marshall, J. S., Hoskin, D. W."Human mast cells induce caspase-independent DNA fragmentation in leukemic T cells". Oncology Reports 10, no. 4 (2003): 1019-1023. https://doi.org/10.3892/or.10.4.1019