Genetic characterization of lung metastases in renal cell carcinoma

  • Authors:
    • Kerstin Junker
    • Jörg Sänger
    • Andreas Schmidt
    • Winfried Hindermann
    • Norbert Presselt
    • Herry Helfritzsch
    • Joerg Schubert
  • View Affiliations

  • Published online on: July 1, 2003     https://doi.org/10.3892/or.10.4.1035
  • Pages: 1035-1038
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Abstract

Prognosis of patients with renal cell carcinoma (RCC) is mainly determined by metastases. The understanding of the metastatic process will give the basis for a differential diagnosis leading to an individual prognosis and to new therapeutical strategies. In order to define specific genetic alterations which are common in renal cancer metastases of the lung, we performed comparative genomic hybridization (CGH) on metastases and in some cases on their related primary renal tumors. For CGH, DNA was isolated from 2 or 5 paraffin sections (5 µm). Tumor and normal (control) DNAs were amplified by DOP-PCR and labeled with biotin-dUTP and digoxigenin-dUTP, respectively. Hybridization and detection were carried out according to standard protocols. In 33 out of 40 metastases, genetic alterations were detected, most frequently these were losses of chromosomes 3p (74%), 8p (31%), 9 or 9q (34%), 14 [26%, 18q (40%) and gains of chromosome 5/5q 34%], 7 (31%) and 12 (26%). Combination of loss of 8p and gain of 8q occurred frequently. The mean number of aberrations per tumor was 8.1 (1-11). The comparison of alterations in related primary and metastatic tumors showed identical alterations in 5 out of 8 cases. This study demonstrates, that lung metastases from renal cell carcinoma are characterized by an accumulation of specific genetic alterations which show a clonal relationship to the related primary tumors.

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July-August 2003
Volume 10 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Junker K, Sänger J, Schmidt A, Hindermann W, Presselt N, Helfritzsch H and Schubert J: Genetic characterization of lung metastases in renal cell carcinoma. Oncol Rep 10: 1035-1038, 2003.
APA
Junker, K., Sänger, J., Schmidt, A., Hindermann, W., Presselt, N., Helfritzsch, H., & Schubert, J. (2003). Genetic characterization of lung metastases in renal cell carcinoma. Oncology Reports, 10, 1035-1038. https://doi.org/10.3892/or.10.4.1035
MLA
Junker, K., Sänger, J., Schmidt, A., Hindermann, W., Presselt, N., Helfritzsch, H., Schubert, J."Genetic characterization of lung metastases in renal cell carcinoma". Oncology Reports 10.4 (2003): 1035-1038.
Chicago
Junker, K., Sänger, J., Schmidt, A., Hindermann, W., Presselt, N., Helfritzsch, H., Schubert, J."Genetic characterization of lung metastases in renal cell carcinoma". Oncology Reports 10, no. 4 (2003): 1035-1038. https://doi.org/10.3892/or.10.4.1035