Blockade of her2/neu decreases VEGF expression but does not alter HIF-1 distribution in experimental Wilms tumor

  • Authors:
    • Akiko Yokoi
    • Kimberly W. McCrudden
    • Jianzhong Huang
    • Eugene S. Kim
    • Samuel Z. Soffer
    • Jason S. Frischer
    • Anna Serur
    • Tamara New
    • Jenny Yuan
    • Mahesh Mansukhani
    • Kathleen O'Toole
    • Darrell J. Yamashiro
    • Jessica J. Kandel
  • View Affiliations

  • Published online on: September 1, 2003     https://doi.org/10.3892/or.10.5.1271
  • Pages: 1271-1274
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Abstract

Her2/neu regulates angiogenesis in human breast cancer, in part by stabilizing hypoxia-inducible factor 1α (HIF-1α), causing accumulation of the HIF-1 heterodimer and thus increasing expression of the proangiogenic cytokine VEGF. Her2/neu has recently been shown to be overexpressed in a subset of Wilms tumors. Using her2/neu (+) and her2/neu (−) Wilms tumor cell lines, we tested the effect of blocking anti-her2/neu antibody in vitro and in vivo. Blocking antibody did not alter VEGF expression in vitro, but decreased expression of VEGF in her2/neu (+) Wilms tumor xenografts. Tumor suppression was less marked than in parallel experiments using agents directly blocking VEGF. HIF-1α immunostaining was not altered in her2/neu (+) xenografts exposed to blocking antibody. These results suggest that her2/neu contributes to Wilms tumor angiogenesis in vivo by regulating VEGF, but other processes may act to rescue HIF-1α and thus to support tumor growth in this system.

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September-October 2003
Volume 10 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yokoi A, McCrudden KW, Huang J, Kim ES, Soffer SZ, Frischer JS, Serur A, New T, Yuan J, Mansukhani M, Mansukhani M, et al: Blockade of her2/neu decreases VEGF expression but does not alter HIF-1 distribution in experimental Wilms tumor. Oncol Rep 10: 1271-1274, 2003
APA
Yokoi, A., McCrudden, K.W., Huang, J., Kim, E.S., Soffer, S.Z., Frischer, J.S. ... Kandel, J.J. (2003). Blockade of her2/neu decreases VEGF expression but does not alter HIF-1 distribution in experimental Wilms tumor. Oncology Reports, 10, 1271-1274. https://doi.org/10.3892/or.10.5.1271
MLA
Yokoi, A., McCrudden, K. W., Huang, J., Kim, E. S., Soffer, S. Z., Frischer, J. S., Serur, A., New, T., Yuan, J., Mansukhani, M., O'Toole, K., Yamashiro, D. J., Kandel, J. J."Blockade of her2/neu decreases VEGF expression but does not alter HIF-1 distribution in experimental Wilms tumor". Oncology Reports 10.5 (2003): 1271-1274.
Chicago
Yokoi, A., McCrudden, K. W., Huang, J., Kim, E. S., Soffer, S. Z., Frischer, J. S., Serur, A., New, T., Yuan, J., Mansukhani, M., O'Toole, K., Yamashiro, D. J., Kandel, J. J."Blockade of her2/neu decreases VEGF expression but does not alter HIF-1 distribution in experimental Wilms tumor". Oncology Reports 10, no. 5 (2003): 1271-1274. https://doi.org/10.3892/or.10.5.1271