GM-CSF secreted by murine adenocarcinoma cells modulates tumor progression and immune activity

  • Authors:
    • M. J. Diament
    • M. I. Stillitani
    • L. Puricelli
    • E. Bal de Kier-Joffe
    • S. Klein
  • View Affiliations

  • Published online on: September 1, 2003     https://doi.org/10.3892/or.10.5.1647
  • Pages: 1647-1652
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Abstract

During tumor development, growth factors may act in autocrine manner stimulating cell proliferation, or in paracrine manner affecting the microenvironment of the tumor and modulating the immune system. Murine mammary adenocarcinoma M3 tumor bearers develop lung metastases and leukocytosis during its evolution. Previously we described that M3 conditioned media enhanced metastasis incidence, when it was inoculated in tumor-operated mice. In the present study we determine that spleen cells from M3 tumor operated mice treated with M3 conditioned media, were able to transfer the capacity to enhance metastasis to other tumor operated mice. Spleen cells have immune suppressor activity that could be reversed by cyclophosfamide treatment. M3 tumor cells secrete GM-CSF, which is able to promote in vitro proliferation of M3 cells as well as spleen cells. This proliferation could be abrogated by the addition of anti-GM-CSF. We report that the GM-CSF secreted by M3 tumor cells had stimulatory activity on M3 tumor cell and lymphocyte proliferation.

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September-October 2003
Volume 10 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Diament MJ, Stillitani MI, Puricelli L, Bal de Kier-Joffe E and Klein S: GM-CSF secreted by murine adenocarcinoma cells modulates tumor progression and immune activity. Oncol Rep 10: 1647-1652, 2003.
APA
Diament, M.J., Stillitani, M.I., Puricelli, L., Bal de Kier-Joffe, E., & Klein, S. (2003). GM-CSF secreted by murine adenocarcinoma cells modulates tumor progression and immune activity. Oncology Reports, 10, 1647-1652. https://doi.org/10.3892/or.10.5.1647
MLA
Diament, M. J., Stillitani, M. I., Puricelli, L., Bal de Kier-Joffe, E., Klein, S."GM-CSF secreted by murine adenocarcinoma cells modulates tumor progression and immune activity". Oncology Reports 10.5 (2003): 1647-1652.
Chicago
Diament, M. J., Stillitani, M. I., Puricelli, L., Bal de Kier-Joffe, E., Klein, S."GM-CSF secreted by murine adenocarcinoma cells modulates tumor progression and immune activity". Oncology Reports 10, no. 5 (2003): 1647-1652. https://doi.org/10.3892/or.10.5.1647