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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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July 2004 Volume 12 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

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July 2004 Volume 12 Issue 1

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Article

Quantitative assessment of HER2/neu expression by real-time PCR and fluorescent in situ hybridization analysis in low-grade osteosarcoma

  • Authors:
    • Woo-In Lee
    • Patrizia Bacchni
    • Franco Bertoni
    • Young Hee Maeng
    • Yong-Koo Park

  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, College of Medicine, Kyung Hee University, Seoul, Korea
  • Pages: 125-128
    |
    Published online on: July 1, 2004
       https://doi.org/10.3892/or.12.1.125
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Abstract

Low-grade osteosarcoma is a rare variant of osteosarcoma. Although malignant, it must be distinguished from conventional osteosarcomas because of its excellent prognosis. Numerous published papers have described the expression of HER2/neu oncogene in osteosarcoma as a poor prognostic factor; however their results are discordant. To address the expression of HER2/neu and to validate the assessment methods of amplification of the HER2/neu oncogene, the authors have employed quantitative real-time PCR and fluorencent in situ hybridization analysis (FISH) in 21 low-grade osteosarcomas. We calculated the quantification of HER2/neu oncogene amplification as the ratio of measured HER2/neu gene/β-globin reference gene in real-time PCR. All 21 cases had amplified signals in the quantitative real-time PCR. However, in the FISH analysis, HER2/neu oncogene amplification was only identified in 26% (5/19). The exact reasons for the discordance between these two methods are unknown; however, variable histological features might play a potential role. In conclusion, as our study showed amplification of HER2/neu oncogene in low-grade osteosarcoma, we assume that expression of HER2/neu is not a poor prognostic factor in low-grade osteosarcoma.

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Copy and paste a formatted citation
Spandidos Publications style
Lee W, Bacchni P, Bertoni F, Maeng YH and Park Y: Quantitative assessment of HER2/neu expression by real-time PCR and fluorescent in situ hybridization analysis in low-grade osteosarcoma. Oncol Rep 12: 125-128, 2004.
APA
Lee, W., Bacchni, P., Bertoni, F., Maeng, Y.H., & Park, Y. (2004). Quantitative assessment of HER2/neu expression by real-time PCR and fluorescent in situ hybridization analysis in low-grade osteosarcoma. Oncology Reports, 12, 125-128. https://doi.org/10.3892/or.12.1.125
MLA
Lee, W., Bacchni, P., Bertoni, F., Maeng, Y. H., Park, Y."Quantitative assessment of HER2/neu expression by real-time PCR and fluorescent in situ hybridization analysis in low-grade osteosarcoma". Oncology Reports 12.1 (2004): 125-128.
Chicago
Lee, W., Bacchni, P., Bertoni, F., Maeng, Y. H., Park, Y."Quantitative assessment of HER2/neu expression by real-time PCR and fluorescent in situ hybridization analysis in low-grade osteosarcoma". Oncology Reports 12, no. 1 (2004): 125-128. https://doi.org/10.3892/or.12.1.125
Copy and paste a formatted citation
x
Spandidos Publications style
Lee W, Bacchni P, Bertoni F, Maeng YH and Park Y: Quantitative assessment of HER2/neu expression by real-time PCR and fluorescent in situ hybridization analysis in low-grade osteosarcoma. Oncol Rep 12: 125-128, 2004.
APA
Lee, W., Bacchni, P., Bertoni, F., Maeng, Y.H., & Park, Y. (2004). Quantitative assessment of HER2/neu expression by real-time PCR and fluorescent in situ hybridization analysis in low-grade osteosarcoma. Oncology Reports, 12, 125-128. https://doi.org/10.3892/or.12.1.125
MLA
Lee, W., Bacchni, P., Bertoni, F., Maeng, Y. H., Park, Y."Quantitative assessment of HER2/neu expression by real-time PCR and fluorescent in situ hybridization analysis in low-grade osteosarcoma". Oncology Reports 12.1 (2004): 125-128.
Chicago
Lee, W., Bacchni, P., Bertoni, F., Maeng, Y. H., Park, Y."Quantitative assessment of HER2/neu expression by real-time PCR and fluorescent in situ hybridization analysis in low-grade osteosarcoma". Oncology Reports 12, no. 1 (2004): 125-128. https://doi.org/10.3892/or.12.1.125
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