Expression of endothelial nitric oxide synthase is induced by estrogen with glycogen synthase 3β phosphorylation in MCF-7 cells

  • Authors:
    • Kaname Nakatani
    • Junichi Horinouchi
    • Yasuyoshi Yabu
    • Hideo Wada
    • Tsutomu Nobori
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/or.12.4.833
  • Pages: 833-836
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Abstract

The endothelial nitric oxide synthase (eNOS) gene is induced by a variety of extracellular signals and NOS plays a key role in many physiological as well as pathological processes, including tumorgenesis. Some studies showed a positive correlation between the level of NOS protein and progression of malignancy in human breast cancer. In this study, we examined eNOS mRNA expression in human breast cancer cell lines. MCF-7 cells, which showed an estrogen receptor positive phenotype, were treated with estradiol or LiCl, a selective inhibitor of glycogen synthase kinase (GSK)-3β. Both estradiol and LiCl enhanced the expression of eNOS mRNA with the phosphorylation of GSK-3β, but not Akt. The induction was completely suppressed by the phosphatidylinositol 3-kinase (PI3-kinase) inhibitor LY294002, but not by PD98059, MEK-1 inhibitor nor rapamycin, p70S6 kinase inhibitor. We conclude that the estradiol-induced eNOS expression is modulated by PI3-kinase-dependent GSK-3β pathway.

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October 2004
Volume 12 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Nakatani K, Horinouchi J, Yabu Y, Wada H and Nobori T: Expression of endothelial nitric oxide synthase is induced by estrogen with glycogen synthase 3β phosphorylation in MCF-7 cells. Oncol Rep 12: 833-836, 2004.
APA
Nakatani, K., Horinouchi, J., Yabu, Y., Wada, H., & Nobori, T. (2004). Expression of endothelial nitric oxide synthase is induced by estrogen with glycogen synthase 3β phosphorylation in MCF-7 cells. Oncology Reports, 12, 833-836. https://doi.org/10.3892/or.12.4.833
MLA
Nakatani, K., Horinouchi, J., Yabu, Y., Wada, H., Nobori, T."Expression of endothelial nitric oxide synthase is induced by estrogen with glycogen synthase 3β phosphorylation in MCF-7 cells". Oncology Reports 12.4 (2004): 833-836.
Chicago
Nakatani, K., Horinouchi, J., Yabu, Y., Wada, H., Nobori, T."Expression of endothelial nitric oxide synthase is induced by estrogen with glycogen synthase 3β phosphorylation in MCF-7 cells". Oncology Reports 12, no. 4 (2004): 833-836. https://doi.org/10.3892/or.12.4.833