Dietary effects of conjugated docosahexaenoic acid on N-methyl-N-nitrosourea-induced mammary carcinogenesis in female Sprague-Dawley rats

  • Authors:
    • Naoyuki Danbara
    • Norihisa Uehara
    • Nobuaki Shikata
    • Hideho Takada
    • Takahiko Hada
    • Airo Tsubura
  • View Affiliations

  • Published online on: November 1, 2004     https://doi.org/10.3892/or.12.5.1079
  • Pages: 1079-1085
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Abstract

The dietary effects of conjugated docosahexaenoic acid (CDHA) were examined in an N-methyl-N-nitrosourea (MNU)-induced rat mammary carcinogenesis model. Female Sprague-Dawley rats were administered 50 mg/kg MNU intraperitoneally at 49 days of age. A powdered AIN-76A diet containing 0, 0.2 or 1.0% CDHA was fed to the rats from 21 to 49 days of age (before MNU; pre-initiation phase) or from 49 days to 40 weeks of age (after MNU; post-initiation phase). Rats were sacrificed when their largest mammary tumor was ≥1 cm in size or when they reached 40 weeks of age. All histologically detected mammary carcinomas were evaluated. In rats that received CDHA after MNU, development of mammary carcinoma ≥1 cm was inhibited, and there was a significant decrease in the final mammary cancer incidence and multiplicity, compared with rats that did not receive CDHA. Consumption of the 0.2% CDHA diet after MNU significantly prolonged latency. Suppression of mammary cancer yield by consumption of a CDHA diet after MNU administration was not dose-dependent. In rats that received CDHA before MNU, suppression of mammary cancer was not observed. These results indicate that CDHA administration in the post-initiation period suppressed mammary carcinogenesis, whereas CDHA administration in the pre-initiation period was ineffective.

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November 2004
Volume 12 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Danbara N, Uehara N, Shikata N, Takada H, Hada T and Tsubura A: Dietary effects of conjugated docosahexaenoic acid on N-methyl-N-nitrosourea-induced mammary carcinogenesis in female Sprague-Dawley rats. Oncol Rep 12: 1079-1085, 2004
APA
Danbara, N., Uehara, N., Shikata, N., Takada, H., Hada, T., & Tsubura, A. (2004). Dietary effects of conjugated docosahexaenoic acid on N-methyl-N-nitrosourea-induced mammary carcinogenesis in female Sprague-Dawley rats. Oncology Reports, 12, 1079-1085. https://doi.org/10.3892/or.12.5.1079
MLA
Danbara, N., Uehara, N., Shikata, N., Takada, H., Hada, T., Tsubura, A."Dietary effects of conjugated docosahexaenoic acid on N-methyl-N-nitrosourea-induced mammary carcinogenesis in female Sprague-Dawley rats". Oncology Reports 12.5 (2004): 1079-1085.
Chicago
Danbara, N., Uehara, N., Shikata, N., Takada, H., Hada, T., Tsubura, A."Dietary effects of conjugated docosahexaenoic acid on N-methyl-N-nitrosourea-induced mammary carcinogenesis in female Sprague-Dawley rats". Oncology Reports 12, no. 5 (2004): 1079-1085. https://doi.org/10.3892/or.12.5.1079