Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays

  • Authors:
    • Aleksandra Ristic-Fira
    • Ivan Petrovic
    • Danijela Todorovic
    • Lela Koricanac
    • Miroslava Vujèic
    • Miroslav Demajo
    • Gabriella Sabini
    • Pablo Cirrone
    • Giacomo Cuttone
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/or.12.6.1323
  • Pages: 1323-1328
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Abstract

The effects of single irradiation with gamma rays and protons on HTB63 human melanoma cell growth were compared. The exponentially growing cells were irradiated with gamma rays or protons using doses ranging from 2-20 Gy. At 48 h of post-irradiation incubation under standard conditions, cell survival and induction of apoptotic cell death were examined. The best effect of the single irradiation with gamma rays was the reduction of cell growth by up to 26% (p=0.048, irradiation vs. control), obtained using the dose of 16 Gy. The same doses of proton irradiation, having energy at the target of 22.6 MeV, significantly inhibited melanoma cell growth. Doses of 12 and 16 Gy of protons provoked growth inhibition of 48.9% (p=0.003, irradiation vs. control) and 51.2% (p=0.012, irradiation vs. control) respectively. Irradiation with 12 and 16 Gy protons, compared to the effects of the same doses of gamma rays, significantly reduced melanoma cell growth (p=0.015 and p=0.028, protons vs. gamma rays, respectively). Estimated RBEs for growth inhibition of HTB63 cells ranged from 1.02 to 1.45. The electrophoretical analyses of DNA samples and flow cytometric evaluation have shown a low percentage of apoptotic cells after both types of irradiation. The better inhibitory effect achieved by protons in contrast to gamma rays, can be explained considering specific physical properties of protons, especially taking into account the highly localized energy deposition (high LET).

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December 2004
Volume 12 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ristic-Fira A, Petrovic I, Todorovic D, Koricanac L, Vujèic M, Demajo M, Sabini G, Cirrone P and Cuttone G: Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays. Oncol Rep 12: 1323-1328, 2004
APA
Ristic-Fira, A., Petrovic, I., Todorovic, D., Koricanac, L., Vujèic, M., Demajo, M. ... Cuttone, G. (2004). Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays. Oncology Reports, 12, 1323-1328. https://doi.org/10.3892/or.12.6.1323
MLA
Ristic-Fira, A., Petrovic, I., Todorovic, D., Koricanac, L., Vujèic, M., Demajo, M., Sabini, G., Cirrone, P., Cuttone, G."Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays". Oncology Reports 12.6 (2004): 1323-1328.
Chicago
Ristic-Fira, A., Petrovic, I., Todorovic, D., Koricanac, L., Vujèic, M., Demajo, M., Sabini, G., Cirrone, P., Cuttone, G."Inactivation of HTB63 human melanoma cells by irradiation with protons and gamma rays". Oncology Reports 12, no. 6 (2004): 1323-1328. https://doi.org/10.3892/or.12.6.1323