PTEN and DMBT1 homozygous deletion and expression in medulloblastomas and supratentorial primitive neuroectodermal tumors

  • Authors:
    • María Del Mar Inda
    • Javier Mercapide
    • Jorge Muñoz
    • Philippe Coullin
    • Giséle Danglot
    • Teresa Tuñon
    • José María Martínez-Peñuela
    • José María Rivera
    • Juan J. Burgos
    • Alain Bernheim
    • Javier S. Castresana
  • View Affiliations

  • Published online on: December 1, 2004     https://doi.org/10.3892/or.12.6.1341
  • Pages: 1341-1347
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Abstract

Medulloblastoma, which accounts for 20-25% of all childhood brain tumors, is defined as a primitive neuroectodermal tumor (PNET) located in the cerebellum. Supratentorial PNET are less frequent than medulloblastoma. But their clinical outcome is worse than in medulloblastomas. Chromosome 10q contains at least 2 tumor suppressor genes that might play a role in brain tumor development: PTEN and DMBT1. The aim of this study was to compare the status of homozygous deletion and expression of PTEN and DMBT1 genes in PNET primary tumor samples and cell lines. Homozygous deletions of PTEN and DMBT1 were studied in 32 paraffin-embedded PNET samples (23 medulloblastomas and 9 supratentorial PNET) and in 7 PNET cell lines, by differential PCR and by FISH. PTEN homozygous losses were demonstrated in 7 medulloblastomas (32%) and in no supratentorial PNET, while homozygous deletions of DMBT1 appeared in 1 supratentorial PNET (20%) and in 7 medulloblastomas (33%). No homozygous deletion of PTEN or DMBT1 was detected in any of the PNET cell lines either by differential PCR or by FISH. Expression study of the 2 genes was performed in the 7 PNET cell lines by RT-PCR. One PNET cell line lacked PTEN and DMBT1 expression, while 2 medulloblastoma cell lines did not express DMBT1. Our results add some positive data to the hypothesis that supratentorial PNETs and medulloblastomas might be genetically different.

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December 2004
Volume 12 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Del Mar Inda M, Mercapide J, Muñoz J, Coullin P, Danglot G, Tuñon T, Martínez-Peñuela JM, Rivera JM, Burgos JJ, Bernheim A, Bernheim A, et al: PTEN and DMBT1 homozygous deletion and expression in medulloblastomas and supratentorial primitive neuroectodermal tumors. Oncol Rep 12: 1341-1347, 2004
APA
Del Mar Inda, M., Mercapide, J., Muñoz, J., Coullin, P., Danglot, G., Tuñon, T. ... Castresana, J.S. (2004). PTEN and DMBT1 homozygous deletion and expression in medulloblastomas and supratentorial primitive neuroectodermal tumors. Oncology Reports, 12, 1341-1347. https://doi.org/10.3892/or.12.6.1341
MLA
Del Mar Inda, M., Mercapide, J., Muñoz, J., Coullin, P., Danglot, G., Tuñon, T., Martínez-Peñuela, J. M., Rivera, J. M., Burgos, J. J., Bernheim, A., Castresana, J. S."PTEN and DMBT1 homozygous deletion and expression in medulloblastomas and supratentorial primitive neuroectodermal tumors". Oncology Reports 12.6 (2004): 1341-1347.
Chicago
Del Mar Inda, M., Mercapide, J., Muñoz, J., Coullin, P., Danglot, G., Tuñon, T., Martínez-Peñuela, J. M., Rivera, J. M., Burgos, J. J., Bernheim, A., Castresana, J. S."PTEN and DMBT1 homozygous deletion and expression in medulloblastomas and supratentorial primitive neuroectodermal tumors". Oncology Reports 12, no. 6 (2004): 1341-1347. https://doi.org/10.3892/or.12.6.1341