Expression of endothelin-1 and endothelin-A and -B receptors in invasive bladder cancer

  • Authors:
    • Christian Wülfing
    • Elke Eltze
    • Hansjürgen Piechota
    • Hassan Abol-Enein
    • Pia Wülfing
    • Marc-Eric Bode
    • Lothar Hertle
    • Karl-Dietrich Sievert
  • View Affiliations

  • Published online on: February 1, 2005     https://doi.org/10.3892/or.13.2.223
  • Pages: 223-228
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Abstract

Overexpression of endothelin-1, endothelin-A- and endothelin-B-receptors has been shown in various human tumors. To assess the role of the ET-axis in bladder cancer, we analyzed its expression in tumor specimens and bladder cancer cell lines. Samples were obtained by radical cystectomy at two urologic institutions. ET-axis expression was investigated by reverse-transcription polymerase chain reaction (RT-PCR) (n=22) and immunohistochemistry (n=42). Additionally, four bladder cancer cell lines were analyzed. RT-PCR analysis for the ET-axis showed positive signals in the majority of cDNA probes. Signals for endothelin-1 (ET-1), endothelin-A-receptor (ETAR) and endothelin-B-receptor (ETBR), as identified semiquantitatively and by densitometry, were found in 22, 22 and 15 of 22 cases, respectively. Immunohistochemistry revealed expression of ET-1, ETA- and ETB-receptor in 18, 29 and 37 of the 42 cases, respectively, whereas normal urothelium was negative. All cell lines expressed ET-1, and all but the RT-112 cell line produced ETAR, whereas no cell line expressed ETBR. The identification of the ET-axis at the mRNA and protein level in the majority of bladder tumor samples suggests a role in the carcinogenesis of bladder cancer. Further studies on regulation of the ET-axis and the future use of selective ETA-receptor inhibitors for targeted molecular therapy in bladder cancer are in progress.

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February 2005
Volume 13 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wülfing C, Eltze E, Piechota H, Abol-Enein H, Wülfing P, Bode M, Hertle L and Sievert K: Expression of endothelin-1 and endothelin-A and -B receptors in invasive bladder cancer. Oncol Rep 13: 223-228, 2005
APA
Wülfing, C., Eltze, E., Piechota, H., Abol-Enein, H., Wülfing, P., Bode, M. ... Sievert, K. (2005). Expression of endothelin-1 and endothelin-A and -B receptors in invasive bladder cancer. Oncology Reports, 13, 223-228. https://doi.org/10.3892/or.13.2.223
MLA
Wülfing, C., Eltze, E., Piechota, H., Abol-Enein, H., Wülfing, P., Bode, M., Hertle, L., Sievert, K."Expression of endothelin-1 and endothelin-A and -B receptors in invasive bladder cancer". Oncology Reports 13.2 (2005): 223-228.
Chicago
Wülfing, C., Eltze, E., Piechota, H., Abol-Enein, H., Wülfing, P., Bode, M., Hertle, L., Sievert, K."Expression of endothelin-1 and endothelin-A and -B receptors in invasive bladder cancer". Oncology Reports 13, no. 2 (2005): 223-228. https://doi.org/10.3892/or.13.2.223