E1AF expression is closely correlated with malignant phenotype of tongue squamous cell carcinoma through activation of MT1-MMP gene promoters

  • Authors:
    • Yuri Izumiyama
    • Yoichi Ohiro
    • Fumihiro Higashino
    • Koichi Yoshida
    • Kazunori Taguchi
    • Satoru Todo
    • Takao Kohgo
    • Yasunori Totsuka
    • Masanobu Shindoh
  • View Affiliations

  • Published online on: April 1, 2005     https://doi.org/10.3892/or.13.4.715
  • Pages: 715-720
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Abstract

E1AF is an ets-oncogene family transcription factor that has been shown to up-regulate multiple MMPs whereas MMP-2, a potent extracellular matrix degrading enzyme, is not up-regulated. We investigated the activation mechanism of MMP-2 in oral squamous cell carcinoma. Chloramphenicol acetyltransferase (CAT) assay was utilized to investigate whether E1AF is able to up-regulate membrane type-1 matrix metalloproteinase (MT1-MMP), which is known to activate MMP-2. Expression of the CAT reporter gene under the control of the MT1-MMP promoter was increased approximately 40-fold by co-transfection with the E1AF expression vector. Real-time RT-PCR study was carried out in 25 patients with tongue squamous cell carcinoma, and the mRNA expression level of E1AF and MT1-MMP was synergistically increased. These results indicate that E1AF positively regulates tran-scription from MT1-MMP genes, which plays an important role in invasion and metastasis of squamous cell carcinoma of the tongue by converting pro-MMP-2 into active-MMP-2.

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April 2005
Volume 13 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Izumiyama Y, Ohiro Y, Higashino F, Yoshida K, Taguchi K, Todo S, Kohgo T, Totsuka Y and Shindoh M: E1AF expression is closely correlated with malignant phenotype of tongue squamous cell carcinoma through activation of MT1-MMP gene promoters. Oncol Rep 13: 715-720, 2005.
APA
Izumiyama, Y., Ohiro, Y., Higashino, F., Yoshida, K., Taguchi, K., Todo, S. ... Shindoh, M. (2005). E1AF expression is closely correlated with malignant phenotype of tongue squamous cell carcinoma through activation of MT1-MMP gene promoters. Oncology Reports, 13, 715-720. https://doi.org/10.3892/or.13.4.715
MLA
Izumiyama, Y., Ohiro, Y., Higashino, F., Yoshida, K., Taguchi, K., Todo, S., Kohgo, T., Totsuka, Y., Shindoh, M."E1AF expression is closely correlated with malignant phenotype of tongue squamous cell carcinoma through activation of MT1-MMP gene promoters". Oncology Reports 13.4 (2005): 715-720.
Chicago
Izumiyama, Y., Ohiro, Y., Higashino, F., Yoshida, K., Taguchi, K., Todo, S., Kohgo, T., Totsuka, Y., Shindoh, M."E1AF expression is closely correlated with malignant phenotype of tongue squamous cell carcinoma through activation of MT1-MMP gene promoters". Oncology Reports 13, no. 4 (2005): 715-720. https://doi.org/10.3892/or.13.4.715