DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60)

  • Authors:
    • Dana Pokorna
    • Noemi Cerovska
    • Michal Smahel
    • Tomas Moravec
    • Viera Ludvikova
    • Jana Mackova
    • Helena Synkova
    • Martina Duskova
    • Pavel Hozak
    • Jiri Veleminsky
  • View Affiliations

  • Published online on: October 1, 2005     https://doi.org/10.3892/or.14.4.1045
  • Pages: 1045-1053
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Abstract

Vaccine strategies for the treatment of human papillomavirus-induced cervical cancer are based mainly on the human papillomavirus 16 E7 (HPV16 E7) oncoprotein. The immunogenicity of the E7 gene has been enhanced by its fusion to many different genes. Here, we linked a short sequence coding for the E7 peptide (aa 44-60) containing immunodominant epitopes for B and T cells to the 3' end of the gene coding for the whole coat protein (CP) of the poty-virus, potato virus A (PVA), and its deleted form (CPdel) with a short C-terminal deletion of 5 amino acids (LGVKG). CP-E7 and CPdel-E7 fusion proteins, just like CP alone, spontaneously assembled into virus-like particles in both procaryotic and eucaryotic cells. The CP-E7 and CPdel-E7 fusion genes induced slightly stronger E7-specific cytotoxic T-lymphocyte responses than the whole E7 gene, although they were still lower than those elicited by the previously constructed fusion gene, Sig/E7GGG/LAMP-1. The E7- and CP-specific antibody responses were not detected in mice vaccinated with CP-E7 and CPdel-E7 fusion genes. The CP-E7 and CPdel-E7 fusion genes protected mice against the development of tumors induced by TC-1 cells producing the E7 antigen and were also effective in the therapeutic setting, i.e. when the vaccination was performed after tumor cell administration. Their antitumor effect was comparable to those of the whole E7 gene and Sig/E7GGG/LAMP-1 fusion gene. There was no relevant difference between immune responses elicited by CP-E7 and CPdel-E7 DNA vaccination.

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October 2005
Volume 14 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Pokorna D, Cerovska N, Smahel M, Moravec T, Ludvikova V, Mackova J, Synkova H, Duskova M, Hozak P, Veleminsky J, Veleminsky J, et al: DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60). Oncol Rep 14: 1045-1053, 2005
APA
Pokorna, D., Cerovska, N., Smahel, M., Moravec, T., Ludvikova, V., Mackova, J. ... Veleminsky, J. (2005). DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60). Oncology Reports, 14, 1045-1053. https://doi.org/10.3892/or.14.4.1045
MLA
Pokorna, D., Cerovska, N., Smahel, M., Moravec, T., Ludvikova, V., Mackova, J., Synkova, H., Duskova, M., Hozak, P., Veleminsky, J."DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60)". Oncology Reports 14.4 (2005): 1045-1053.
Chicago
Pokorna, D., Cerovska, N., Smahel, M., Moravec, T., Ludvikova, V., Mackova, J., Synkova, H., Duskova, M., Hozak, P., Veleminsky, J."DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60)". Oncology Reports 14, no. 4 (2005): 1045-1053. https://doi.org/10.3892/or.14.4.1045