Radiation-induced cell death is independent of the apoptotic signals mediated by death-associated protein kinase in human cervical squamous cell carcinoma cells

  • Authors:
    • Tetsuji Tanaka
    • Tao Bai
    • Kazunori Yukawa
    • Naohiko Umesaki
  • View Affiliations

  • Published online on: October 1, 2005     https://doi.org/10.3892/or.14.4.949
  • Pages: 949-955
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Abstract

Death-associated protein kinase (DAPK) was originally identified as a positive mediator of interferon-γ (IFNγ)-induced apoptosis in cervical cancer cells, and interferons have been reported to enhance radiosensitivity in various types of squamous cell carcinoma. To examine whether DAPK can regulate cancer cell radiosensitivity, we investigated DAPK expression and radiosensitivity in human cancer cell lines, including the cervical squamous cell carcinoma cell line, ME180, which is both radiosensitive and IFNγ-sensitive. Of the 5 human cancer cell lines examined, ME180 cells were the most radiosensitive, but their level of DAPK protein expression was undetectable by western blotting. A comparative study of ME180 cells with 2 other uterine cancer cell lines, HHUA and HOKUG, revealed no significant relationships between cellular radiosensitivity and DAPK protein expression or hypermethylation of the DAPK promoter CpG island. INFγ dose-dependently inhibited ME180 cell proliferation, but did not induce any cell death. IFNγ significantly enhanced the radiosensitivity of ME180 cells with a slight increase in DAPK protein expression, while irradiation significantly reduced their sensitivity to the growth-inhibitory signals of INFγ. Analyses of 6 monoclonal cisplatin-resistant subclones established from ME180 cells revealed that all 6 were significantly more radioresistant than the parent ME180 cells without any change in the DAPK protein expression. These results indicate that DAPK does not regulate radiation-induced cell death and that it cannot be either a target molecule for radiotherapy with gene therapy or a prognostic marker for cervical cancer patients treated with radiotherapy.

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October 2005
Volume 14 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Tanaka T, Bai T, Yukawa K and Umesaki N: Radiation-induced cell death is independent of the apoptotic signals mediated by death-associated protein kinase in human cervical squamous cell carcinoma cells. Oncol Rep 14: 949-955, 2005
APA
Tanaka, T., Bai, T., Yukawa, K., & Umesaki, N. (2005). Radiation-induced cell death is independent of the apoptotic signals mediated by death-associated protein kinase in human cervical squamous cell carcinoma cells. Oncology Reports, 14, 949-955. https://doi.org/10.3892/or.14.4.949
MLA
Tanaka, T., Bai, T., Yukawa, K., Umesaki, N."Radiation-induced cell death is independent of the apoptotic signals mediated by death-associated protein kinase in human cervical squamous cell carcinoma cells". Oncology Reports 14.4 (2005): 949-955.
Chicago
Tanaka, T., Bai, T., Yukawa, K., Umesaki, N."Radiation-induced cell death is independent of the apoptotic signals mediated by death-associated protein kinase in human cervical squamous cell carcinoma cells". Oncology Reports 14, no. 4 (2005): 949-955. https://doi.org/10.3892/or.14.4.949