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Complex formations involving both SP-1 and SP-3 at the transcriptional regulatory sequence correlate with the activation of the Keratin 14 gene in human oral squamous cell carcinoma cells

  • Authors:
    • Shuri Ohkura
    • Nobuo Kondoh
    • Akiyuki Hada
    • Masaaki Arai
    • Yutaka Yamazaki
    • Masanobu Sindoh
    • Masayuki Takahashi
    • Isamu Matsumoto
    • Mikio Yamamoto

  • View Affiliations

  • Published online on: December 1, 2005     https://doi.org/10.3892/or.14.6.1577
  • Pages: 1577-1581
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Abstract

We have previously reported that significantly higher levels of Keratin 14 (Ker-14) was observed in oral squamous cell carcinoma (OSCC) and severely dysplastic tissues, whereas this expression was reversed in hyperplasia and in mild to moderate dysplasia. In this study, the mechanism of Keratin 14 activation in oral squamous cell carcinoma (OSCC) cell lines (HSC-2, HSC-3 and Ca9-22) was investigated. Reporter analysis demonstrated that an upstream region (-1759/-1629) accounted for efficient promoter activity. Furthermore, electromobility sift and supershift assay demonstrated that interactions of the SP-1/SP-3 complex at the elements resided in -1737/-1702 and -1680/-1652 and may be essential for this activation in OSCC cells.

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December 2005
Volume 14 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Impact Factor: 4.2
  • Ranked Oncology
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  • CiteScore: 8.4
  • CiteScore Rank: Q1: #68/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.786
  • SCImago Journal Rank (SJR): 0.812
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Spandidos Publications style
Ohkura S, Kondoh N, Hada A, Arai M, Yamazaki Y, Sindoh M, Takahashi M, Matsumoto I and Yamamoto M: Complex formations involving both SP-1 and SP-3 at the transcriptional regulatory sequence correlate with the activation of the Keratin 14 gene in human oral squamous cell carcinoma cells. Oncol Rep 14: 1577-1581, 2005
APA
Ohkura, S., Kondoh, N., Hada, A., Arai, M., Yamazaki, Y., Sindoh, M. ... Yamamoto, M. (2005). Complex formations involving both SP-1 and SP-3 at the transcriptional regulatory sequence correlate with the activation of the Keratin 14 gene in human oral squamous cell carcinoma cells. Oncology Reports, 14, 1577-1581. https://doi.org/10.3892/or.14.6.1577
MLA
Ohkura, S., Kondoh, N., Hada, A., Arai, M., Yamazaki, Y., Sindoh, M., Takahashi, M., Matsumoto, I., Yamamoto, M."Complex formations involving both SP-1 and SP-3 at the transcriptional regulatory sequence correlate with the activation of the Keratin 14 gene in human oral squamous cell carcinoma cells". Oncology Reports 14.6 (2005): 1577-1581.
Chicago
Ohkura, S., Kondoh, N., Hada, A., Arai, M., Yamazaki, Y., Sindoh, M., Takahashi, M., Matsumoto, I., Yamamoto, M."Complex formations involving both SP-1 and SP-3 at the transcriptional regulatory sequence correlate with the activation of the Keratin 14 gene in human oral squamous cell carcinoma cells". Oncology Reports 14, no. 6 (2005): 1577-1581. https://doi.org/10.3892/or.14.6.1577