Molecular cloning of rHAUSP encoding a deubiquitinating enzyme in rat testis

  • Authors:
    • Kwang-Hyun Baek
    • Hye-Jin Lee
    • Myung-Sun Kim
    • Yong-Soo Kim
    • Minu Seong
    • Eung-Ji Lee
    • Min-Young Lee
  • View Affiliations

  • Published online on: January 1, 2006     https://doi.org/10.3892/or.15.1.173
  • Pages: 173-177
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Abstract

The tumor suppressor protein p53 is stabilized by the herpes-virus-associated ubiquitin-specific protease (HAUSP), a deubiquitinating enzyme. We previously isolated and characterized a mouse orthologue of HAUSP, mHAUSP. In this study, we have identified a rat orthologue of HAUSP, rHAUSP, from the rat testis by RT-PCR using primers used for cloning mHAUSP. rHAUSP cDNA encodes 3,312 bp and 1,103 amino acids with a molecular weight of approximately 135 kDa containing highly conserved Cys, Asp (I), His, and Asn/Asp (II) domains characteristic of the ubiquitin-specific processing proteases. pI value of rHAUSP is 5.31. In vivo and in vitro deubiquitinating enzyme assays demonstrated that rHAUSP has deubiquitinating enzymatic activity. The over-expression of rHAUSP induced cell death of cervical adenocarcinoma cells.

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January 2006
Volume 15 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Baek K, Lee H, Kim M, Kim Y, Seong M, Lee E and Lee M: Molecular cloning of rHAUSP encoding a deubiquitinating enzyme in rat testis. Oncol Rep 15: 173-177, 2006
APA
Baek, K., Lee, H., Kim, M., Kim, Y., Seong, M., Lee, E., & Lee, M. (2006). Molecular cloning of rHAUSP encoding a deubiquitinating enzyme in rat testis. Oncology Reports, 15, 173-177. https://doi.org/10.3892/or.15.1.173
MLA
Baek, K., Lee, H., Kim, M., Kim, Y., Seong, M., Lee, E., Lee, M."Molecular cloning of rHAUSP encoding a deubiquitinating enzyme in rat testis". Oncology Reports 15.1 (2006): 173-177.
Chicago
Baek, K., Lee, H., Kim, M., Kim, Y., Seong, M., Lee, E., Lee, M."Molecular cloning of rHAUSP encoding a deubiquitinating enzyme in rat testis". Oncology Reports 15, no. 1 (2006): 173-177. https://doi.org/10.3892/or.15.1.173