Down-regulation of MLL-AF9, MLL and MYC expression is not obligatory for monocyte-macrophage maturation in AML-M5 cell lines carrying t(9;11)(p22;q23)

  • Authors:
    • Vincenzo Martino
    • Roberto Tonelli
    • Luca Montemurro
    • Monica Franzoni
    • Flora Marino
    • Raffaella Fazzina
    • Andrea Pession
  • View Affiliations

  • Published online on: January 1, 2006     https://doi.org/10.3892/or.15.1.207
  • Pages: 207-211
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The MLL-AF9 oncogene originates from the translocation t(9;11)(p22;q23), which is mainly associated with monocytic acute myeloid leukaemia (AML-M5; FAB-classification). In AML-M5 THP-1 cells carrying t(9;11) (p22;q23) and expressing MLL-AF9, we previously showed that MLL-AF9 expression is down-regulated during monocyte-macrophage maturation. We have subsequently observed that in a ‘rapid-growing’ variant of the THP-1 cell line (THP-1-R) MLL-AF9 down-regulation does not occur. MLL fusion proteins (including MLL-AF9) deregulate MYC transactivation activity, and both presence and absence of MYC down-regulation have been reported during monocyte-macrophage maturation in THP-1 cells. In the present study, we analyze the expression patterns of MLL-AF9, MLL wild-type and MYC after induction of monocyte-macrophage terminal differentiation in the AML-M5 cell lines, THP-1, THP-1-R, Mono-Mac-6 (MM6) and MOLM-13, all of which carry t(9;11)(p22;q23) and express MLL-AF9. RT-PCR analysis indicated that down-regulation of MLL-AF9, MLL or MYC is not necessary to abolish malignant phenotypes by induction of terminal monocyte-macrophage differentiation in leukaemic cells carrying t(9;11)(p22;q23).

Related Articles

Journal Cover

January 2006
Volume 15 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Martino V, Tonelli R, Montemurro L, Franzoni M, Marino F, Fazzina R and Pession A: Down-regulation of MLL-AF9, MLL and MYC expression is not obligatory for monocyte-macrophage maturation in AML-M5 cell lines carrying t(9;11)(p22;q23). Oncol Rep 15: 207-211, 2006
APA
Martino, V., Tonelli, R., Montemurro, L., Franzoni, M., Marino, F., Fazzina, R., & Pession, A. (2006). Down-regulation of MLL-AF9, MLL and MYC expression is not obligatory for monocyte-macrophage maturation in AML-M5 cell lines carrying t(9;11)(p22;q23). Oncology Reports, 15, 207-211. https://doi.org/10.3892/or.15.1.207
MLA
Martino, V., Tonelli, R., Montemurro, L., Franzoni, M., Marino, F., Fazzina, R., Pession, A."Down-regulation of MLL-AF9, MLL and MYC expression is not obligatory for monocyte-macrophage maturation in AML-M5 cell lines carrying t(9;11)(p22;q23)". Oncology Reports 15.1 (2006): 207-211.
Chicago
Martino, V., Tonelli, R., Montemurro, L., Franzoni, M., Marino, F., Fazzina, R., Pession, A."Down-regulation of MLL-AF9, MLL and MYC expression is not obligatory for monocyte-macrophage maturation in AML-M5 cell lines carrying t(9;11)(p22;q23)". Oncology Reports 15, no. 1 (2006): 207-211. https://doi.org/10.3892/or.15.1.207