Plasma membrane Ca2+ ATPase isoform 1 down-regulated in human oral cancer

  • Authors:
    • Kengo Saito
    • Katsuhiro Uzawa
    • Yosuke Endo
    • Yoshikuni Kato
    • Dai Nakashima
    • Katsunori Ogawara
    • Masashi Shiba
    • Hiroki Bukawa
    • Hidetaka Yokoe
    • Hideki Tanzawa
  • View Affiliations

  • Published online on: January 1, 2006     https://doi.org/10.3892/or.15.1.49
  • Pages: 49-55
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Abstract

The plasma membrane Ca2+ ATPase (PMCA) is an essential regulator of free intracellular calcium. Recent studies have reported aberrant expression of the PMCA1 gene, a member of the PMCA family, in several cancer cell types. To elucidate the contribution of PMCA1 to oral carcinogenesis, we analyzed genetic and epigenetic changes and mRNA and protein expression in primary oral squamous cell carcinomas (OSCCs), oral premalignant lesions (OPLs), and OSCC-derived cell lines. The PMCA1 gene was epigenetically inactivated, but not mutated in the eight OSCC-derived cell lines tested. In clinical samples, frequent down-regulation of PMCA1 protein expression was found not only in primary OSCCs (43%), but also in OPLs (40%). Real-time quantitative reverse transcriptase-polymerase chain reaction data were consistent with the protein expression status. These results suggest that inactivation of the PMCA1 gene is a frequent and early event during oral carcinogenesis, and gene expression may be regulated by an epigenetic mechanism.

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January 2006
Volume 15 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Saito K, Uzawa K, Endo Y, Kato Y, Nakashima D, Ogawara K, Shiba M, Bukawa H, Yokoe H, Tanzawa H, Tanzawa H, et al: Plasma membrane Ca2+ ATPase isoform 1 down-regulated in human oral cancer. Oncol Rep 15: 49-55, 2006.
APA
Saito, K., Uzawa, K., Endo, Y., Kato, Y., Nakashima, D., Ogawara, K. ... Tanzawa, H. (2006). Plasma membrane Ca2+ ATPase isoform 1 down-regulated in human oral cancer. Oncology Reports, 15, 49-55. https://doi.org/10.3892/or.15.1.49
MLA
Saito, K., Uzawa, K., Endo, Y., Kato, Y., Nakashima, D., Ogawara, K., Shiba, M., Bukawa, H., Yokoe, H., Tanzawa, H."Plasma membrane Ca2+ ATPase isoform 1 down-regulated in human oral cancer". Oncology Reports 15.1 (2006): 49-55.
Chicago
Saito, K., Uzawa, K., Endo, Y., Kato, Y., Nakashima, D., Ogawara, K., Shiba, M., Bukawa, H., Yokoe, H., Tanzawa, H."Plasma membrane Ca2+ ATPase isoform 1 down-regulated in human oral cancer". Oncology Reports 15, no. 1 (2006): 49-55. https://doi.org/10.3892/or.15.1.49