mHFE7A, a newly identified monoclonal antibody to Fas, induces apoptosis in human melanoma cells in vitro and delays the growth of melanoma xenotransplants

  • Authors:
    • Kenichi Hiramoto
    • Madoka Inui
    • Takahiko Kamei
    • Kenya Okumura
    • Minoru Nakase
    • Toshiro Tagawa
  • View Affiliations

  • Published online on: February 1, 2006     https://doi.org/10.3892/or.15.2.409
  • Pages: 409-415
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The Fas receptor is a potentially valuable therapeutic target in cancer treatment. However, the clinical application of antibodies directed to this target is hindered by their serious side effects in vivo, including liver toxicity. One murine monoclonal antibody, mHFE7A, binds both to human Fas and murine Fas, without inducing any obvious side effects. However, the potential therapeutic effects of mHFE7A are unclear in human cancer cells or tumors. Here, we determined whether mHFE7A could induce apoptosis in vitro, and assessed its effects on major apoptotic pathways in a human melanoma cell line, MMN9. We also investigated its anti-cancer effects on transplanted melanoma MMN9 tumors in BALB/c nude mice. Treatment of mHFE7A cross-linking with Ig induced cell death similar to CH-11 treatment. Apoptosis induced by mHFE7A was defined by Hoechst 33342 DNA staining and DNA fragmentation assay. Furthermore, mHFE7A-mediated apoptosis was dependent on activation of a caspase signaling cascade involving caspases-8 and -3. Administration of mHFE7A also delayed the growth of melanoma xenografts. Thus, we provide the first evidence that the murine anti-Fas monoclonal antibody, mHFE7A, induces apoptosis of human malignant melanoma cells in vitro and is anti-tumorigenic in vivo.

Related Articles

Journal Cover

February 2006
Volume 15 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hiramoto K, Inui M, Kamei T, Okumura K, Nakase M and Tagawa T: mHFE7A, a newly identified monoclonal antibody to Fas, induces apoptosis in human melanoma cells in vitro and delays the growth of melanoma xenotransplants. Oncol Rep 15: 409-415, 2006
APA
Hiramoto, K., Inui, M., Kamei, T., Okumura, K., Nakase, M., & Tagawa, T. (2006). mHFE7A, a newly identified monoclonal antibody to Fas, induces apoptosis in human melanoma cells in vitro and delays the growth of melanoma xenotransplants. Oncology Reports, 15, 409-415. https://doi.org/10.3892/or.15.2.409
MLA
Hiramoto, K., Inui, M., Kamei, T., Okumura, K., Nakase, M., Tagawa, T."mHFE7A, a newly identified monoclonal antibody to Fas, induces apoptosis in human melanoma cells in vitro and delays the growth of melanoma xenotransplants". Oncology Reports 15.2 (2006): 409-415.
Chicago
Hiramoto, K., Inui, M., Kamei, T., Okumura, K., Nakase, M., Tagawa, T."mHFE7A, a newly identified monoclonal antibody to Fas, induces apoptosis in human melanoma cells in vitro and delays the growth of melanoma xenotransplants". Oncology Reports 15, no. 2 (2006): 409-415. https://doi.org/10.3892/or.15.2.409