Induction of G2/M arrest and inhibition of cyclooxygenase-2 activity by curcumin in human bladder cancer T24 cells

  • Authors:
    • Cheol Park
    • Gi Young Kim
    • Gun Do Kim
    • Byung Tae Choi
    • Yeong-Min Park
    • Yung Hyun Choi
  • View Affiliations

  • Published online on: May 1, 2006     https://doi.org/10.3892/or.15.5.1225
  • Pages: 1225-1231
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Abstract

Curcumin, a polyphenol compound derived from Curcuma longa Linn, has been recognized as a promising anti-cancer drug due to its multiple properties including anti-inflammatory, anti-oxidant and anti-carcinogenic activities. To elucidate the mechanisms by which curcumin inhibits human bladder carcinoma T24 cell proliferation, we tested the effects of curcumin on specific cell cycle pathways and on the expression of cyclooxygenases (COXs). Curcumin inhibited the growth of T24 cells and induced G2/M arrest in a concentration-dependent manner, effects associated with the down-regulation of cyclin A and up-regulation of cyclin-dependent kinase (Cdk) inhibitor p21 (WAF1/CIP1). However, other G2/M regulatory molecules, such as cyclin A, Cdc2, Cdk2, Wee1 and Cdc25C, were not modulated by curcumin treatment. Furthermore, curcumin decreased the levels of COX-2 mRNA and protein expression without significant changes in the levels of COX-1, which correlated with a decrease in prostaglandin E2 (PGE2) synthesis. These observations suggest that curcumin may have therapeutic potential for bladder cancer patients.

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May 2006
Volume 15 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Park C, Kim GY, Kim GD, Choi BT, Park Y and Choi YH: Induction of G2/M arrest and inhibition of cyclooxygenase-2 activity by curcumin in human bladder cancer T24 cells. Oncol Rep 15: 1225-1231, 2006
APA
Park, C., Kim, G.Y., Kim, G.D., Choi, B.T., Park, Y., & Choi, Y.H. (2006). Induction of G2/M arrest and inhibition of cyclooxygenase-2 activity by curcumin in human bladder cancer T24 cells. Oncology Reports, 15, 1225-1231. https://doi.org/10.3892/or.15.5.1225
MLA
Park, C., Kim, G. Y., Kim, G. D., Choi, B. T., Park, Y., Choi, Y. H."Induction of G2/M arrest and inhibition of cyclooxygenase-2 activity by curcumin in human bladder cancer T24 cells". Oncology Reports 15.5 (2006): 1225-1231.
Chicago
Park, C., Kim, G. Y., Kim, G. D., Choi, B. T., Park, Y., Choi, Y. H."Induction of G2/M arrest and inhibition of cyclooxygenase-2 activity by curcumin in human bladder cancer T24 cells". Oncology Reports 15, no. 5 (2006): 1225-1231. https://doi.org/10.3892/or.15.5.1225