PEA3 cooperates with c-Jun in regulation of HER2/neu transcription

  • Authors:
    • Koshi Matsui
    • Kazuhito Sugimori
    • Hiraku Motomura
    • Naoko Ejiri
    • Kazuhiro Tsukada
    • Isao Kitajima
  • View Affiliations

  • Published online on: July 1, 2006     https://doi.org/10.3892/or.16.1.153
  • Pages: 153-158
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Abstract

HER2/neu overexpressing breast tumors exhibit an increase in polyomavirus enhancer activator 3 (PEA3) expression. We examined the relationship between HER2/neu transcriptional activation and PEA3 in cooperation with c-Jun. HER2/neu promoter activity was decreased by deleting PEA3 binding site, and was downregulated when the PEA3 binding site was mutated. PEA3 and c-Jun each weakly enhanced luciferase expression of the HER2/neu promoter. However, the HER2/neu promoter response to PEA3 was considerably enhanced by c-Jun. Thus, we examined the interaction of PEA3 with c-Jun by the two-hybrid system, the transcriptional activity of PEA3 was specifically enhanced by c-Jun. When PEA3, c-Jun and coactivator p300 were cotransfected in MCF7 cells, the transcriptional activity of HER2/neu was increased by up to 20-fold. PEA3 and c-Jun-induced transcription of HER2/neu promoter was repressed by cotransfection of the dominant negative of p300. These results suggest that PEA3 and c-Jun stimulated synergistically the HER2/neu gene transcription with p300.

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July 2006
Volume 16 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Matsui K, Sugimori K, Motomura H, Ejiri N, Tsukada K and Kitajima I: PEA3 cooperates with c-Jun in regulation of HER2/neu transcription. Oncol Rep 16: 153-158, 2006
APA
Matsui, K., Sugimori, K., Motomura, H., Ejiri, N., Tsukada, K., & Kitajima, I. (2006). PEA3 cooperates with c-Jun in regulation of HER2/neu transcription. Oncology Reports, 16, 153-158. https://doi.org/10.3892/or.16.1.153
MLA
Matsui, K., Sugimori, K., Motomura, H., Ejiri, N., Tsukada, K., Kitajima, I."PEA3 cooperates with c-Jun in regulation of HER2/neu transcription". Oncology Reports 16.1 (2006): 153-158.
Chicago
Matsui, K., Sugimori, K., Motomura, H., Ejiri, N., Tsukada, K., Kitajima, I."PEA3 cooperates with c-Jun in regulation of HER2/neu transcription". Oncology Reports 16, no. 1 (2006): 153-158. https://doi.org/10.3892/or.16.1.153