Alterations in the Smad4 gene in hamster pancreatic duct adenocarcinomas and established cell lines

  • Authors:
    • Kyoko Shimizu
    • Tsukasa Kitahashi
    • Hiromasa Fujii
    • Masahiro Tsutsumi
    • Toshio Mori
    • Kanya Honoki
    • Toshifumi Tsujiuchi
  • View Affiliations

  • Published online on: July 1, 2006     https://doi.org/10.3892/or.16.1.85
  • Pages: 85-89
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Alterations of the Smad4 gene, identified as a mediator of the transforming growth factor-β pathway, were investigated in hamster pancreatic duct adenocarcinomas (PDAs) and established cell lines. Female Syrian golden hamsters received 70 mg/kg of N-nitrosobis(2-oxopropyl)amine (BOP) followed by repeated exposure to an augmentation pressure regimen consisting of a choline-deficient diet combined with DL-ethionine then L-methionine and a further administration of 20 mg/kg BOP. A total of 12 PDAs obtained 10 weeks after beginning the experiment and three cell lines established from subcutaneously transplantable PDAs in syngeneic hamsters were examined for mutations using reverse transcription-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP) analysis. A mutation was detected in only one PDA (1/12, 8.3%) in the form of an ACC to ATC (Thr to IIe) transition at codon 73; none were detected in the three cell lines. No reduced or increased expression of the Smad4 gene was detected in any case using real-time quantitative RT-PCR. These results suggest that the Smad4 gene might play a role in limited fraction of BOP-induced pancreatic duct carcinogenesis in hamsters.

Related Articles

Journal Cover

July 2006
Volume 16 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Shimizu K, Kitahashi T, Fujii H, Tsutsumi M, Mori T, Honoki K and Tsujiuchi T: Alterations in the Smad4 gene in hamster pancreatic duct adenocarcinomas and established cell lines. Oncol Rep 16: 85-89, 2006
APA
Shimizu, K., Kitahashi, T., Fujii, H., Tsutsumi, M., Mori, T., Honoki, K., & Tsujiuchi, T. (2006). Alterations in the Smad4 gene in hamster pancreatic duct adenocarcinomas and established cell lines. Oncology Reports, 16, 85-89. https://doi.org/10.3892/or.16.1.85
MLA
Shimizu, K., Kitahashi, T., Fujii, H., Tsutsumi, M., Mori, T., Honoki, K., Tsujiuchi, T."Alterations in the Smad4 gene in hamster pancreatic duct adenocarcinomas and established cell lines". Oncology Reports 16.1 (2006): 85-89.
Chicago
Shimizu, K., Kitahashi, T., Fujii, H., Tsutsumi, M., Mori, T., Honoki, K., Tsujiuchi, T."Alterations in the Smad4 gene in hamster pancreatic duct adenocarcinomas and established cell lines". Oncology Reports 16, no. 1 (2006): 85-89. https://doi.org/10.3892/or.16.1.85