AKAPs competing peptide HT31 disrupts the inhibitory effect of PKA on RhoA activity

  • Authors:
    • Ying Wang
    • Yongchang Chen
    • Min Chen
    • Wenrong Xu
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/or.16.4.755
  • Pages: 755-761
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

A-kinase can inhibit RhoA activation through phosphorylating ser188 of RhoA. AKAP is a novel protein that can target PKA to different subcellular compartment. Evidence has been presented that PKA anchorage by AKAP is important for the kinase to exert its function. This study analyzed the role of PKA anchorage in PKA-induced antagonism against RhoA activity and function. The cells transfected with pcDNA HT31wt/mut were treated with LPA and/or CPT-cAMP. The amount of GTP-RhoA and phosphorylation of RhoA was detected by Western blotting with specific antibodies. The formation of stress fiber was visualized under fluorescent microscope. The gene expression activity was analyzed by luciferase reporter gene assay. The motility and the anchorage-independent growth assays were carried out with stably transfected cells expressing the AKAP inhibitory peptide HT31. The results showed that HT31 not only blocked the PKA-induced phosphorylation of RhoA but also prevented the PKA-induced inhibition on RhoA activation. The disruption of PKA anchorage abolished its inhibition on the LPA-induced expression of reporter gene SRE-luciferase. The ability of PKA to antagonize the LPA-induced stress fiber formation was partly impaired upon the disruption of the PKA anchorage. The control of PKA on migration and the proliferation excited by LPA disappeared in stably transfected cells highly expressing HT31. The results revealed that PKA anchorage was necessary for the kinase to exert its inhibitory effect on RhoA activation and RhoA-dependent biological activities.

Related Articles

Journal Cover

October 2006
Volume 16 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Chen Y, Chen M and Xu W: AKAPs competing peptide HT31 disrupts the inhibitory effect of PKA on RhoA activity. Oncol Rep 16: 755-761, 2006
APA
Wang, Y., Chen, Y., Chen, M., & Xu, W. (2006). AKAPs competing peptide HT31 disrupts the inhibitory effect of PKA on RhoA activity. Oncology Reports, 16, 755-761. https://doi.org/10.3892/or.16.4.755
MLA
Wang, Y., Chen, Y., Chen, M., Xu, W."AKAPs competing peptide HT31 disrupts the inhibitory effect of PKA on RhoA activity". Oncology Reports 16.4 (2006): 755-761.
Chicago
Wang, Y., Chen, Y., Chen, M., Xu, W."AKAPs competing peptide HT31 disrupts the inhibitory effect of PKA on RhoA activity". Oncology Reports 16, no. 4 (2006): 755-761. https://doi.org/10.3892/or.16.4.755