Regulatory roles of cell surface sialylation in sphingolipid-induced cell death in human B cell lymphoma

  • Authors:
    • Osamu Suzuki
    • Yoshihiro Nozawa
    • Masafumi Abe
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/or.16.4.851
  • Pages: 851-857
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Abstract

Sphingolipid metabolites are important regulators of cell growth and death. In the present study, we examined the function of cell surface sialic acid in exogenous sphingosine-1-phosphate (S-1-P) or sphingosine-induced cell death. HBL-2 human diffuse large B cell lymphoma cells were incubated with or without Vibrio Cholerae neuraminidase followed by S-1-P or sphingosine. Flow cytometric analysis using Limax flavus agglutinin, a sialic acid-specific lectin, showed that sialylated glycoconjugates are present on the surface of HBL-2 cells and that they were removed by neuraminidase. In addition, the pretreatment with neuraminidase enhanced S-1-P- and sphingosine-induced cell death, an effect that was not dependent on caspase activation. Furthermore, the cell death induced by S-1-P and sphingosine was morphologically distinct from apoptosis. We further examined S-1-P-induced cell death in two clones of HBL-8 Burkitt lymphoma cells with different amounts of cell surface sialic acid. Clone 3G3, which is hypersialylated, was less sensitive to S-1-P than the 3D2 clone, which is hyposialylated, suggesting that the extent of surface sialylation influences the sensitivity to S-1-P. In conclusion, S-1-P and sphingosine induce cell death, and the sensitivity of human B lymphoma cells to these agents appears to depend on the amount of sialic acid on their cell surfaces.

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October 2006
Volume 16 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Suzuki O, Nozawa Y and Abe M: Regulatory roles of cell surface sialylation in sphingolipid-induced cell death in human B cell lymphoma. Oncol Rep 16: 851-857, 2006
APA
Suzuki, O., Nozawa, Y., & Abe, M. (2006). Regulatory roles of cell surface sialylation in sphingolipid-induced cell death in human B cell lymphoma. Oncology Reports, 16, 851-857. https://doi.org/10.3892/or.16.4.851
MLA
Suzuki, O., Nozawa, Y., Abe, M."Regulatory roles of cell surface sialylation in sphingolipid-induced cell death in human B cell lymphoma". Oncology Reports 16.4 (2006): 851-857.
Chicago
Suzuki, O., Nozawa, Y., Abe, M."Regulatory roles of cell surface sialylation in sphingolipid-induced cell death in human B cell lymphoma". Oncology Reports 16, no. 4 (2006): 851-857. https://doi.org/10.3892/or.16.4.851