Preferentially enhanced gene expression from a synthetic human telomerase reverse transcriptase promoter in human cancer cells

  • Authors:
    • Sung Jin Kim
    • Han Saem Lee
    • June Ho Shin
    • Chul Geun Kim
    • Sunjoo Jeong
    • Keerang Park
    • Han Choe
    • Heuiran Lee
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/or.16.5.975
  • Pages: 975-979
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Abstract

Although the human telomerase reverse transcriptase (hTERT) promoter can regulate cancer-specific genes, it is generally too weak to be effective. We therefore attempted to improve the potency of synthetic hTERT promoters by fusing the core element (E) of the hTERT promoter (H) and the tripartite leader sequence (T) from human adenovirus 5 in a combinatorial manner. To determine the potential as cancer-specific promoters, we measured luciferase activity driven by the chimeric hTERT promoters in human cancer cells. Among various constructs, the E3-H-T promoter induced the strongest luciferase activity in all the tested cancer cells. SK-Hep1 and Hela cells experienced 1000- and 11-fold higher expression than the basic hTERT promoter, respectively. Relative to the SV40 universal promoter, the E3-H-T promoter led to higher levels of gene expression. Using EMSA, we found that the hTERT enhancer region was specifically bound to c-Myc and Sp1. Thus, the data suggest that the E3-H-T promoter with up-regulated cancer-specific gene expression could be useful in cancer gene therapy.

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November 2006
Volume 16 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kim SJ, Lee HS, Shin JH, Kim CG, Jeong S, Park K, Choe H and Lee H: Preferentially enhanced gene expression from a synthetic human telomerase reverse transcriptase promoter in human cancer cells. Oncol Rep 16: 975-979, 2006
APA
Kim, S.J., Lee, H.S., Shin, J.H., Kim, C.G., Jeong, S., Park, K. ... Lee, H. (2006). Preferentially enhanced gene expression from a synthetic human telomerase reverse transcriptase promoter in human cancer cells. Oncology Reports, 16, 975-979. https://doi.org/10.3892/or.16.5.975
MLA
Kim, S. J., Lee, H. S., Shin, J. H., Kim, C. G., Jeong, S., Park, K., Choe, H., Lee, H."Preferentially enhanced gene expression from a synthetic human telomerase reverse transcriptase promoter in human cancer cells". Oncology Reports 16.5 (2006): 975-979.
Chicago
Kim, S. J., Lee, H. S., Shin, J. H., Kim, C. G., Jeong, S., Park, K., Choe, H., Lee, H."Preferentially enhanced gene expression from a synthetic human telomerase reverse transcriptase promoter in human cancer cells". Oncology Reports 16, no. 5 (2006): 975-979. https://doi.org/10.3892/or.16.5.975