Malignant transformation of the cultured human normal biliary tract epithelial cells induced by hepatitis C virus core protein

  • Authors:
    • Ru-Fu Chen
    • Zhi-Hua Li
    • Ran-Yi Liu
    • Xian-He Kong
    • Qi-Bin Tang
    • Jie Wang
  • View Affiliations

  • Published online on: January 1, 2007     https://doi.org/10.3892/or.17.1.105
  • Pages: 105-110
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

High level expression of hepatitis C virus core protein (HCV-C) was detected in hilar cholangiocarcinoma tissues in our previous studies. This protein played an important role in the process of cancer cell inversion and proliferation, by some direct and indirect effects on certain genes. Based on this observation, we investigated the effect of HCV-C on human normal biliary epithelial (hBE) cell transformation and tumor development. Plasmid pHCV-C encoding the gene of HCV core protein was constructed and transfected into hBE cells. The expression and the biological effect of HCV-C in HCV-C gene-modified hBE cells were determined in vitro and in vivo. The clone formation rates of hBE cells transfected with pHCV-C, pcdna3.1 and mock-transfected cells were 36, 2.5 and 1.5%, respectively. Tumor developed in 7 of 7 nude mice after incubated with pHCV-C transfected hBE cells, while no tumor appeared in mice injected with pcdna3.1- and mock-transfected hBE cells. To investigate the possible mechanism of malignant transformation, we further studied the telomerase activity and human telomerase reverse transcriptase (hTERT) expression in pHCV-C transfected hBE cells. The elevated expression of hTERT was confirmed by RT-PCR, immunocytochemistry (ICC) and Western blot analysis, which in turn elevated the telomerase activity, confirmed by TRAP-ELISA. These results indicated that HCV-C protein could participate in malignant transformation of human normal biliary epithelial cells and induce cholangiocarcinoma tumorigenesis, and the activation of telomerase was one of the possible mechanisms.

Related Articles

Journal Cover

January 2007
Volume 17 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chen R, Li Z, Liu R, Kong X, Tang Q and Wang J: Malignant transformation of the cultured human normal biliary tract epithelial cells induced by hepatitis C virus core protein. Oncol Rep 17: 105-110, 2007
APA
Chen, R., Li, Z., Liu, R., Kong, X., Tang, Q., & Wang, J. (2007). Malignant transformation of the cultured human normal biliary tract epithelial cells induced by hepatitis C virus core protein. Oncology Reports, 17, 105-110. https://doi.org/10.3892/or.17.1.105
MLA
Chen, R., Li, Z., Liu, R., Kong, X., Tang, Q., Wang, J."Malignant transformation of the cultured human normal biliary tract epithelial cells induced by hepatitis C virus core protein". Oncology Reports 17.1 (2007): 105-110.
Chicago
Chen, R., Li, Z., Liu, R., Kong, X., Tang, Q., Wang, J."Malignant transformation of the cultured human normal biliary tract epithelial cells induced by hepatitis C virus core protein". Oncology Reports 17, no. 1 (2007): 105-110. https://doi.org/10.3892/or.17.1.105