Gene profile analysis of colorectal cancer cell lines by cDNA macroarray

  • Authors:
    • Noboru Saito
    • Shingo Kameoka
    • Ryuji Furukawa
  • View Affiliations

  • Published online on: May 1, 2007     https://doi.org/10.3892/or.17.5.1061
  • Pages: 1061-1065
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Abstract

In order to identify high-risk groups of patients with cancer, understanding the mechanisms of invasion and metastasis from the viewpoint of gene expression is required; in particular, the changes in gene expression as tumours gain the ability to metastasise. Using a rat model of metastatic colorectal cancer (CRC), we determined the expression profiles of CRC cells that metastasised to the liver and the lung. In the hepatopulmonary metastasis model, metastasising ability increased with successive transplanted cell line generations. No significant differences in cellular proliferation ability were seen between the original cell line and succeeding metastatic cell lines. Analysis using cDNA macroarray showed that metastasising ability was associated with increased expression of integrin β4, γ-catenin, Smad 7, Bax, Bcl-2, c-fos and TGF-α, and a particularly marked increased expression of TGF-β, PDGFb, Cdk4 and Rho B. Expression of both Rho GDIβ and Gelsolin was reduced. These results suggest that high metastasising ability does not derive from a single gene, but rather through accumulated changes in the expression of several different genes.

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May 2007
Volume 17 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Saito N, Kameoka S and Furukawa R: Gene profile analysis of colorectal cancer cell lines by cDNA macroarray. Oncol Rep 17: 1061-1065, 2007.
APA
Saito, N., Kameoka, S., & Furukawa, R. (2007). Gene profile analysis of colorectal cancer cell lines by cDNA macroarray. Oncology Reports, 17, 1061-1065. https://doi.org/10.3892/or.17.5.1061
MLA
Saito, N., Kameoka, S., Furukawa, R."Gene profile analysis of colorectal cancer cell lines by cDNA macroarray". Oncology Reports 17.5 (2007): 1061-1065.
Chicago
Saito, N., Kameoka, S., Furukawa, R."Gene profile analysis of colorectal cancer cell lines by cDNA macroarray". Oncology Reports 17, no. 5 (2007): 1061-1065. https://doi.org/10.3892/or.17.5.1061