Detection of activated K-ras in non-small cell lung cancer by membrane array: A comparison with direct sequencing

  • Authors:
    • Inn-Wen Chong
    • Mei-Yin Chang
    • Chau-Chyun Sheu
    • Cheng-Yuan Wang
    • Jhi-Jhu Hwang
    • Ming-Shyan Huang
    • Shiu-Ru Lin
  • View Affiliations

  • Published online on: July 1, 2007     https://doi.org/10.3892/or.18.1.17
  • Pages: 17-24
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Abstract

The ability to detect K-ras oncogene may provide additional information for the management of patients with non-small cell lung cancer (NSCLC). In the present study, we detected the K-ras oncogene in 76 patients with NSCLC by two methods: direct sequencing of K-ras in tumor tissues and membrane array detection of the gene overexpression specific for activated K-ras in peripheral blood. The results showed that 28 (36.8%) of the 76 Taiwanese NSCLC patients had K-ras mutations, with a frequency of 36.4% (20/55) in adenocarcinomas and 38.1% (8/21) in squamous cell carcinomas. The K-ras mutations were more frequently found in smokers than in non-smokers (51.4 vs. 24.4%, P=0.015). The incidences of K-ras mutation in the subgroups of non-smokers and squamous cell carcinomas are relatively higher in Taiwan than in other countries. On the other hand, the membrane array method could positively detect circulating activated K-ras in all of the 27 NSCLC patients with K-ras mutations at codons 12, 13 and 61, and in 4 of the 48 patients with wild-type K-ras. Our results suggest that the K-ras oncogene membrane array serves as a sensitive and convenient tool for the detection of K-ras oncogene, and therefore, has a great potential for clinical applications.

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July 2007
Volume 18 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chong I, Chang M, Sheu C, Wang C, Hwang J, Huang M and Lin S: Detection of activated K-ras in non-small cell lung cancer by membrane array: A comparison with direct sequencing. Oncol Rep 18: 17-24, 2007
APA
Chong, I., Chang, M., Sheu, C., Wang, C., Hwang, J., Huang, M., & Lin, S. (2007). Detection of activated K-ras in non-small cell lung cancer by membrane array: A comparison with direct sequencing. Oncology Reports, 18, 17-24. https://doi.org/10.3892/or.18.1.17
MLA
Chong, I., Chang, M., Sheu, C., Wang, C., Hwang, J., Huang, M., Lin, S."Detection of activated K-ras in non-small cell lung cancer by membrane array: A comparison with direct sequencing". Oncology Reports 18.1 (2007): 17-24.
Chicago
Chong, I., Chang, M., Sheu, C., Wang, C., Hwang, J., Huang, M., Lin, S."Detection of activated K-ras in non-small cell lung cancer by membrane array: A comparison with direct sequencing". Oncology Reports 18, no. 1 (2007): 17-24. https://doi.org/10.3892/or.18.1.17