Effect of 41°C and 43°C on cisplatin radiosensitization in two human carcinoma cell lines with different sensitivities for cisplatin

  • Authors:
    • Judith W.J. Bergs
    • Jaap Haveman
    • Rosemarie Ten Cate
    • Jan Paul Medema
    • Nicolaas A.P. Franken
    • Chris van Bree
  • View Affiliations

  • Published online on: July 1, 2007     https://doi.org/10.3892/or.18.1.219
  • Pages: 219-226
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Abstract

The effect of trimodality treatment consisting of hyperthermia, cisplatin and radiation was investigated in two cell lines with different sensitivities to cisplatin. Hyperthermia treatment was performed for 1 h at 41°C and 43°C in order to compare the effects of the two temperatures. Clonogenic assays were performed with cisplatin-sensitive SiHa human cervical carcinoma and cisplatin-resistant SW-1573 human lung carcinoma cell lines. Cells were treated with various combinations of hyperthermia, cisplatin and radiation. Radiation was performed after 1 h of simultaneous hyperthermia and cisplatin treatment. Cisplatin exposure was for 1 h or continuous without refreshment of the cisplatin-containing medium. SiHa cells were more sensitive to cisplatin than SW-1573 cells. Hyperthermia at 41°C decreased survival in SW-1573 cells but was not cytotoxic in SiHa cells. Hyperthermia at 43°C decreased survival dramatically in both cell lines with SiHa being the most sensitive. The addition of hyperthermia at 41°C and 43°C to cisplatin treatment led to enhanced cell kill in both cell lines compared with cisplatin alone. Radiosensitization was observed after continuous but not after 1 h of cisplatin treatment. Hyperthermia at 43°C increased radiosensitivity whereas hyperthermia at 41°C did not. A combination of 41°C hyperthermia with continuous cisplatin treatment had an additive effect on SW-1573 cells but enhanced cisplatin radiosensitivity of SiHa cells. In SW-1573 cells trimodality treatment using 43°C hyperthermia enhances cisplatin radiosensitivity. We conclude that hyperthermia at 43°C enhances cisplatin-induced radiosensitization in both cisplatin-sensitive and -resistant cell lines. Hyperthermia at 41°C was also able to increase cisplatin-induced radiosensitivity but only in the cisplatin-sensitive SiHa cell line.

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July 2007
Volume 18 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Bergs JW, Haveman J, Ten Cate R, Medema JP, Franken NA and van Bree C: Effect of 41°C and 43°C on cisplatin radiosensitization in two human carcinoma cell lines with different sensitivities for cisplatin. Oncol Rep 18: 219-226, 2007
APA
Bergs, J.W., Haveman, J., Ten Cate, R., Medema, J.P., Franken, N.A., & van Bree, C. (2007). Effect of 41°C and 43°C on cisplatin radiosensitization in two human carcinoma cell lines with different sensitivities for cisplatin. Oncology Reports, 18, 219-226. https://doi.org/10.3892/or.18.1.219
MLA
Bergs, J. W., Haveman, J., Ten Cate, R., Medema, J. P., Franken, N. A., van Bree, C."Effect of 41°C and 43°C on cisplatin radiosensitization in two human carcinoma cell lines with different sensitivities for cisplatin". Oncology Reports 18.1 (2007): 219-226.
Chicago
Bergs, J. W., Haveman, J., Ten Cate, R., Medema, J. P., Franken, N. A., van Bree, C."Effect of 41°C and 43°C on cisplatin radiosensitization in two human carcinoma cell lines with different sensitivities for cisplatin". Oncology Reports 18, no. 1 (2007): 219-226. https://doi.org/10.3892/or.18.1.219