Inhibition of tumor growth through suppression of angiogenesis by brain-specific angiogenesis inhibitor 1 gene transfer in murine renal cell carcinoma

  • Authors:
    • Shigetaka Kudo
    • Ryuichiro Konda
    • Wataru Obara
    • Daisuke Kudo
    • Kenzaburo Tani
    • Yusuke Nakamura
    • Tomoaki Fujioka
  • View Affiliations

  • Published online on: October 1, 2007     https://doi.org/10.3892/or.18.4.785
  • Pages: 785-791
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Abstract

This study was designed to elucidate the therapeutic effect of transfering the brain-specific angiogenesis inhibitor 1 (BAI1) gene to a mouse renal cell carcinoma cell line (Renca). Female BALB/c mice were inoculated subcutaneously with wild-type Renca (Renca/Wild) cells or Renca cells transfected with the BAI-1 (Renca/BAI-1) or LacZ (Renca/LacZ) gene. Tumor growth was observed every other day from 3 to 35 days after implantation. Moreover, the intratumoral injection of the adenovirus vector containing the gene encoding BAI1 was conducted at two-day intervals from 11 to 31 days after implantation of the Renca/Wild or Renca/BAI1 tumor. Tumor blood flow was measured by colorimetric angiogenesis assay (CAA). The concentration of the vascular endothelial growth factor (VEGF) in the cell culture supernatants was determined by enzyme-linked immunoassay. The size of the Renca/BAI1 tumor was significantly (p<0.01) suppressed compared to the Renca/Wild and Renca/LacZ tumors 21 days after tumor implantation. The injection of the BAI1 viral vector at 2-day intervals significantly inhibited the growth of both the Renca/Wild and Renca/BAI1 tumors. The blood volume measured by CAA and microvessel density was significantly lower in the Renca/BAI1 than in the Renca/Wild and Renca/LacZ tumors (p<0.01 and p<0.05, respectively). A significant (p<0.01) reduction in VEGF concentration in the supernatant was demonstrated in the Renca/BAI1 compared with the Renca/Wild and Renca/LacZ cell cultures. These observations suggest that the transfer of the BAI1 gene to Renca can suppress the tumor growth via the inhibition of angiogenesis. The down-regulation of VEGF production in tumor cells contributes to this anti-tumor effect.

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October 2007
Volume 18 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kudo S, Konda R, Obara W, Kudo D, Tani K, Nakamura Y and Fujioka T: Inhibition of tumor growth through suppression of angiogenesis by brain-specific angiogenesis inhibitor 1 gene transfer in murine renal cell carcinoma. Oncol Rep 18: 785-791, 2007.
APA
Kudo, S., Konda, R., Obara, W., Kudo, D., Tani, K., Nakamura, Y., & Fujioka, T. (2007). Inhibition of tumor growth through suppression of angiogenesis by brain-specific angiogenesis inhibitor 1 gene transfer in murine renal cell carcinoma. Oncology Reports, 18, 785-791. https://doi.org/10.3892/or.18.4.785
MLA
Kudo, S., Konda, R., Obara, W., Kudo, D., Tani, K., Nakamura, Y., Fujioka, T."Inhibition of tumor growth through suppression of angiogenesis by brain-specific angiogenesis inhibitor 1 gene transfer in murine renal cell carcinoma". Oncology Reports 18.4 (2007): 785-791.
Chicago
Kudo, S., Konda, R., Obara, W., Kudo, D., Tani, K., Nakamura, Y., Fujioka, T."Inhibition of tumor growth through suppression of angiogenesis by brain-specific angiogenesis inhibitor 1 gene transfer in murine renal cell carcinoma". Oncology Reports 18, no. 4 (2007): 785-791. https://doi.org/10.3892/or.18.4.785