The relationship between the down-regulation of DNA-PKcs or Ku70 and the chemosensitization in human cervical carcinoma cell line HeLa

  • Authors:
    • Xiaoyu Tian
    • Gang Chen
    • Hui Xing
    • Danhui Weng
    • Yunhong Guo
    • Ding Ma
  • View Affiliations

  • Published online on: October 1, 2007     https://doi.org/10.3892/or.18.4.927
  • Pages: 927-932
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Abstract

The aim of this study was to clarify the function of non-homologous end-joining (NHEJ) in tumorigenesis and chemoresistance, and to explore the potential of DNA-PK as a target of reversal of chemoresistance and enhancing the sensitivity of cells to chemotherapeutic agents. Plasmid vectors pSIREN-Ku70shRNA and pSIREN-DNA-PKcssh-RNA, which coded small interfering RNA of Ku70 and DNA-PKcs, were constructed and transfected into human cervical cancer cell line HeLa. The relationship between the down-regulation of Ku70 or DNA-PKcs and tumor cell proliferation and the sensitivity of cells to chemotherapeutic agents were analyzed. Down-regulation of Ku70 and DNA-PKcs expression inhibited cell proliferation, and increased cell apoptosis in DDP-treated HeLa cells. DNA-PK might play an important role in drug resistance, and inhibition of the DNA-PK expression suppressed the growth of tumor cells and enhanced the sensitivity of cells to chemotherapeutic agents.

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October 2007
Volume 18 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Tian X, Chen G, Xing H, Weng D, Guo Y and Ma D: The relationship between the down-regulation of DNA-PKcs or Ku70 and the chemosensitization in human cervical carcinoma cell line HeLa. Oncol Rep 18: 927-932, 2007
APA
Tian, X., Chen, G., Xing, H., Weng, D., Guo, Y., & Ma, D. (2007). The relationship between the down-regulation of DNA-PKcs or Ku70 and the chemosensitization in human cervical carcinoma cell line HeLa. Oncology Reports, 18, 927-932. https://doi.org/10.3892/or.18.4.927
MLA
Tian, X., Chen, G., Xing, H., Weng, D., Guo, Y., Ma, D."The relationship between the down-regulation of DNA-PKcs or Ku70 and the chemosensitization in human cervical carcinoma cell line HeLa". Oncology Reports 18.4 (2007): 927-932.
Chicago
Tian, X., Chen, G., Xing, H., Weng, D., Guo, Y., Ma, D."The relationship between the down-regulation of DNA-PKcs or Ku70 and the chemosensitization in human cervical carcinoma cell line HeLa". Oncology Reports 18, no. 4 (2007): 927-932. https://doi.org/10.3892/or.18.4.927