Expression of hypoxia inducible factor-1α (HIF-1α) and glucose transporter-1 (GLUT-1) in ovarian adenocarcinomas: Difference in hypoxic status depending on histological character

  • Authors:
    • Masanori Yasuda
    • Masaki Miyazawa
    • Mariko Fujita
    • Hiroshi Kajiwara
    • Tetsuji Iida
    • Takeshi Hirasawa
    • Toshinari Muramatsu
    • Masaru Murakami
    • Mikio Mikami
    • Kaoru Saitoh
    • Michio Shimizu
    • Susumu Takekoshi
    • R. Yoshiyuki Osamura
  • View Affiliations

  • Published online on: January 1, 2008     https://doi.org/10.3892/or.19.1.111
  • Pages: 111-116
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Abstract

The expression of hypoxia inducible factor-1α (HIF-1α) and glucose transporter-1 (GLUT-1) was immunohistochemically analyzed in ovarian adenocarcinomas with the aim of elucidating whether hypoxic status is associated with histological type or structural character. The following ovarian adenocarcinomas were used: serous adenocarcinoma (SEA), 21 cases; mucinous adenocarcinoma (MUA), 19 cases; endometrioid adenocarcinoma (ENA), 16 cases; clear cell adenocarcinoma (CLA), 19 cases. High-level expression (3+) of HIF-1α was observed in 100% of SEAs, 58% of MUAs, 100% of ENAs and 89% of CLAs, and high-level expression of GLUT-1 in 76% of SEAs, 26% of MUAs, 50% of ENAs and 67% of CLAs. Heterogeneous or localized staining was relatively evident for GLUT-1. Immunohistochemical profiles were in accord with the immunoblotting and mRNA levels of both markers. ELISA for the detection of active HIF-1 demonstrated that HIF-1 is strongly activated in SEAs, ENAs and CLAs as compared to MUAs. Our results show that GLUT-1 overexpression is to some extent regulated by HIF-1α and is also strongly associated with histological features, i.e., papillary or stratified structure accompanied by little or no vascular stroma. In conclusion, hypoxic status differs according to the histological type of ovarian adenocarcinoma and the micro-environmental conditions of each type.

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January 2008
Volume 19 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yasuda M, Miyazawa M, Fujita M, Kajiwara H, Iida T, Hirasawa T, Muramatsu T, Murakami M, Mikami M, Saitoh K, Saitoh K, et al: Expression of hypoxia inducible factor-1α (HIF-1α) and glucose transporter-1 (GLUT-1) in ovarian adenocarcinomas: Difference in hypoxic status depending on histological character. Oncol Rep 19: 111-116, 2008
APA
Yasuda, M., Miyazawa, M., Fujita, M., Kajiwara, H., Iida, T., Hirasawa, T. ... Osamura, R.Y. (2008). Expression of hypoxia inducible factor-1α (HIF-1α) and glucose transporter-1 (GLUT-1) in ovarian adenocarcinomas: Difference in hypoxic status depending on histological character. Oncology Reports, 19, 111-116. https://doi.org/10.3892/or.19.1.111
MLA
Yasuda, M., Miyazawa, M., Fujita, M., Kajiwara, H., Iida, T., Hirasawa, T., Muramatsu, T., Murakami, M., Mikami, M., Saitoh, K., Shimizu, M., Takekoshi, S., Osamura, R. Y."Expression of hypoxia inducible factor-1α (HIF-1α) and glucose transporter-1 (GLUT-1) in ovarian adenocarcinomas: Difference in hypoxic status depending on histological character". Oncology Reports 19.1 (2008): 111-116.
Chicago
Yasuda, M., Miyazawa, M., Fujita, M., Kajiwara, H., Iida, T., Hirasawa, T., Muramatsu, T., Murakami, M., Mikami, M., Saitoh, K., Shimizu, M., Takekoshi, S., Osamura, R. Y."Expression of hypoxia inducible factor-1α (HIF-1α) and glucose transporter-1 (GLUT-1) in ovarian adenocarcinomas: Difference in hypoxic status depending on histological character". Oncology Reports 19, no. 1 (2008): 111-116. https://doi.org/10.3892/or.19.1.111