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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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February 2008 Volume 19 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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February 2008 Volume 19 Issue 2

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Article

Increased numbers of endothelial progenitor cells in peripheral blood and tumor specimens in non-small cell lung cancer: A methodological challenge and an ongoing debate on the clinical relevance

  • Authors:
    • Andreas Pircher
    • Christian Matthias Kähler
    • Sergej Skvortsov
    • Margit Dlaska
    • Gerold Kawaguchi
    • Thomas Schmid
    • Eberhard Gunsilius
    • Wolfgang Hilbe
  • View Affiliations / Copyright

    Affiliations: Department of General Internal Medicine, Medical University Innsbruck, A-6020 Innsbruck, Austria
  • Pages: 345-352
    |
    Published online on: February 1, 2008
       https://doi.org/10.3892/or.19.2.345
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Abstract

Bone marrow-derived endothelial progenitor cells (EPC) play an important role in neovascularisation and tumor growth. However, the clinical relevance of EPCs on blood vessel formation in non-small cell lung cancer (NSCLC) is unclear. EPC numbers in circulation are very low and therefore their detection is technically challenging. In the present study, 10 NSCLC patients and 5 healthy controls were included. Patients underwent blood analyses before and after surgery. EPCs were isolated from whole blood by magnetic cell sorting to CD34 (MACS). Afterwards, FACS analyses using antibodies against CD133, CD34, VEGFR2 and CD45 and and immunocytological staining to CD133 on cytospins (MCA) were performed. Cryostat sections of tumor samples were stained for CD133, CD31 and cytokeratin A7. Serum levels of the vascular endothelial growth factor (VEGF) were quantified by sandwich ELISA. Compared to the control group NSCLC patients showed significantly elevated EPC counts and VEGF levels in peripheral blood before and after surgery. From a methodological point of view, the tested procedure (MCA) was validated as compared to the standard FACS analyses (CD34+/VEGFR2+). MCA proved to have a very high sensitivity and even allowed the identification of singular positive EPCs.

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Copy and paste a formatted citation
Spandidos Publications style
Pircher A, Kähler CM, Skvortsov S, Dlaska M, Kawaguchi G, Schmid T, Gunsilius E and Hilbe W: Increased numbers of endothelial progenitor cells in peripheral blood and tumor specimens in non-small cell lung cancer: A methodological challenge and an ongoing debate on the clinical relevance. Oncol Rep 19: 345-352, 2008.
APA
Pircher, A., Kähler, C.M., Skvortsov, S., Dlaska, M., Kawaguchi, G., Schmid, T. ... Hilbe, W. (2008). Increased numbers of endothelial progenitor cells in peripheral blood and tumor specimens in non-small cell lung cancer: A methodological challenge and an ongoing debate on the clinical relevance. Oncology Reports, 19, 345-352. https://doi.org/10.3892/or.19.2.345
MLA
Pircher, A., Kähler, C. M., Skvortsov, S., Dlaska, M., Kawaguchi, G., Schmid, T., Gunsilius, E., Hilbe, W."Increased numbers of endothelial progenitor cells in peripheral blood and tumor specimens in non-small cell lung cancer: A methodological challenge and an ongoing debate on the clinical relevance". Oncology Reports 19.2 (2008): 345-352.
Chicago
Pircher, A., Kähler, C. M., Skvortsov, S., Dlaska, M., Kawaguchi, G., Schmid, T., Gunsilius, E., Hilbe, W."Increased numbers of endothelial progenitor cells in peripheral blood and tumor specimens in non-small cell lung cancer: A methodological challenge and an ongoing debate on the clinical relevance". Oncology Reports 19, no. 2 (2008): 345-352. https://doi.org/10.3892/or.19.2.345
Copy and paste a formatted citation
x
Spandidos Publications style
Pircher A, Kähler CM, Skvortsov S, Dlaska M, Kawaguchi G, Schmid T, Gunsilius E and Hilbe W: Increased numbers of endothelial progenitor cells in peripheral blood and tumor specimens in non-small cell lung cancer: A methodological challenge and an ongoing debate on the clinical relevance. Oncol Rep 19: 345-352, 2008.
APA
Pircher, A., Kähler, C.M., Skvortsov, S., Dlaska, M., Kawaguchi, G., Schmid, T. ... Hilbe, W. (2008). Increased numbers of endothelial progenitor cells in peripheral blood and tumor specimens in non-small cell lung cancer: A methodological challenge and an ongoing debate on the clinical relevance. Oncology Reports, 19, 345-352. https://doi.org/10.3892/or.19.2.345
MLA
Pircher, A., Kähler, C. M., Skvortsov, S., Dlaska, M., Kawaguchi, G., Schmid, T., Gunsilius, E., Hilbe, W."Increased numbers of endothelial progenitor cells in peripheral blood and tumor specimens in non-small cell lung cancer: A methodological challenge and an ongoing debate on the clinical relevance". Oncology Reports 19.2 (2008): 345-352.
Chicago
Pircher, A., Kähler, C. M., Skvortsov, S., Dlaska, M., Kawaguchi, G., Schmid, T., Gunsilius, E., Hilbe, W."Increased numbers of endothelial progenitor cells in peripheral blood and tumor specimens in non-small cell lung cancer: A methodological challenge and an ongoing debate on the clinical relevance". Oncology Reports 19, no. 2 (2008): 345-352. https://doi.org/10.3892/or.19.2.345
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