Response of normal and colon cancer epithelial cells to TNF-family apoptotic inducers

  • Authors:
    • Jirina Hofmanová
    • Alena Vaculová
    • Martina Hyzd'Alová
    • Alois Kozubík
  • View Affiliations

  • Published online on: February 1, 2008     https://doi.org/10.3892/or.19.2.567
  • Pages: 567-573
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Abstract

We compared the response of normal (FHC) and cancer (HT-29) human colon epithelial cells to the important apoptotic inducers TNF-α, anti-Fas antibody and TNF-related apoptosis inducing ligand (TRAIL). The two cell lines did not respond to TNF-α (15 ng/ml), expressed a limited sensitivity to anti-Fas antibody (200 ng/ml) and a different response to TRAIL (100 ng/ml). We studied apoptosis with regard to the changes at the receptor level (DR, DcR and FLIP) and at the level of mitochondria (Bid protein cleavage, Apo2.7 protein expression and caspase-9 activation). Two different approaches were used to sensitize the cells to TRAIL-induced apoptosis: inhibition of protein synthesis (cycloheximide, CHX) and inhibition of the pro-survival MEK/ERK pathway (U0126). While the two cell lines were markedly sensitized to all three TNF family members by CHX, a different degree of response (especially for TRAIL) was obtained when inhibition of the MEK/ERK pathway was achieved. TRAIL-induced apoptosis was significantly enhanced by U0126 co-treatment in the HT-29 cells, but not in the FHC cells. The most significant differences between the HT-29 and FHC cells co-treated with TRAIL and U0126 were demonstrated with regard to the involvement of the mitochondrial apoptotic pathway, suggesting its importance in the regulation of cell sensitivity to the TRAIL-induced apoptosis.

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February 2008
Volume 19 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Hofmanová J, Vaculová A, Hyzd'Alová M and Kozubík A: Response of normal and colon cancer epithelial cells to TNF-family apoptotic inducers. Oncol Rep 19: 567-573, 2008
APA
Hofmanová, J., Vaculová, A., Hyzd'Alová, M., & Kozubík, A. (2008). Response of normal and colon cancer epithelial cells to TNF-family apoptotic inducers. Oncology Reports, 19, 567-573. https://doi.org/10.3892/or.19.2.567
MLA
Hofmanová, J., Vaculová, A., Hyzd'Alová, M., Kozubík, A."Response of normal and colon cancer epithelial cells to TNF-family apoptotic inducers". Oncology Reports 19.2 (2008): 567-573.
Chicago
Hofmanová, J., Vaculová, A., Hyzd'Alová, M., Kozubík, A."Response of normal and colon cancer epithelial cells to TNF-family apoptotic inducers". Oncology Reports 19, no. 2 (2008): 567-573. https://doi.org/10.3892/or.19.2.567