Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity

  • Authors:
    • Xiaobin Qian
    • Jing Cheng
    • Aiping Chen
    • Ying Wang
    • Yan Tao
    • Jianping Cao
    • Zhenqing Feng
  • View Affiliations

  • Published online on: February 1, 2008     https://doi.org/10.3892/or.19.2.575
  • Pages: 575-581
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Abstract

Human telomerase reverse transcriptase (hTERT) is an attractive target for cancer gene therapy. However, the poor inhibition of telomerase and a time lag between the inhibition and arrest of cell proliferation limits its use in cancer gene therapy. RNA interference (RNAi) has been proposed as a potential technique for the treatment of cancer with a long-term gene silencing response induced by short hairpin RNA (shRNA). To investigate the long-term effects of telomerase inhibition through the down-regulation of the hTERT gene and the potential role of hTERT in cancer gene therapy, we constructed an shRNA-directed hTERT-expressing vector and introduced it into SGC-7901 cells. A population of cells that stably expressed the shRNA was selected by G418 and continuously cultured in a medium with half the antibiotic concentration for 3 months and the anti-proliferation effects of shRNA-targeted hTERT were then detected. The results showed that shRNA-targeted hTERT significantly inhibited cell proliferation and increased cell apoptosis by down-regulating hTERT expression, thus decreasing telomerase activity. These findings suggest that shRNA-targeted hTERT has long-term anti-proliferation effects on SGC-7901 cells, and it is a potential approach in telomerase-based gene therapy.

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February 2008
Volume 19 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Qian X, Cheng J, Chen A, Wang Y, Tao Y, Cao J and Feng Z: Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity. Oncol Rep 19: 575-581, 2008
APA
Qian, X., Cheng, J., Chen, A., Wang, Y., Tao, Y., Cao, J., & Feng, Z. (2008). Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity. Oncology Reports, 19, 575-581. https://doi.org/10.3892/or.19.2.575
MLA
Qian, X., Cheng, J., Chen, A., Wang, Y., Tao, Y., Cao, J., Feng, Z."Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity". Oncology Reports 19.2 (2008): 575-581.
Chicago
Qian, X., Cheng, J., Chen, A., Wang, Y., Tao, Y., Cao, J., Feng, Z."Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity". Oncology Reports 19, no. 2 (2008): 575-581. https://doi.org/10.3892/or.19.2.575