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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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February 2008 Volume 19 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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February 2008 Volume 19 Issue 2

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Article

Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity

  • Authors:
    • Xiaobin Qian
    • Jing Cheng
    • Aiping Chen
    • Ying Wang
    • Yan Tao
    • Jianping Cao
    • Zhenqing Feng

  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Nanjing Medical University, Shanghai, P.R. China
  • Pages: 575-581
    |
    Published online on: February 1, 2008
       https://doi.org/10.3892/or.19.2.575
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Abstract

Human telomerase reverse transcriptase (hTERT) is an attractive target for cancer gene therapy. However, the poor inhibition of telomerase and a time lag between the inhibition and arrest of cell proliferation limits its use in cancer gene therapy. RNA interference (RNAi) has been proposed as a potential technique for the treatment of cancer with a long-term gene silencing response induced by short hairpin RNA (shRNA). To investigate the long-term effects of telomerase inhibition through the down-regulation of the hTERT gene and the potential role of hTERT in cancer gene therapy, we constructed an shRNA-directed hTERT-expressing vector and introduced it into SGC-7901 cells. A population of cells that stably expressed the shRNA was selected by G418 and continuously cultured in a medium with half the antibiotic concentration for 3 months and the anti-proliferation effects of shRNA-targeted hTERT were then detected. The results showed that shRNA-targeted hTERT significantly inhibited cell proliferation and increased cell apoptosis by down-regulating hTERT expression, thus decreasing telomerase activity. These findings suggest that shRNA-targeted hTERT has long-term anti-proliferation effects on SGC-7901 cells, and it is a potential approach in telomerase-based gene therapy.

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Copy and paste a formatted citation
Spandidos Publications style
Qian X, Cheng J, Chen A, Wang Y, Tao Y, Cao J and Feng Z: Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity. Oncol Rep 19: 575-581, 2008.
APA
Qian, X., Cheng, J., Chen, A., Wang, Y., Tao, Y., Cao, J., & Feng, Z. (2008). Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity. Oncology Reports, 19, 575-581. https://doi.org/10.3892/or.19.2.575
MLA
Qian, X., Cheng, J., Chen, A., Wang, Y., Tao, Y., Cao, J., Feng, Z."Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity". Oncology Reports 19.2 (2008): 575-581.
Chicago
Qian, X., Cheng, J., Chen, A., Wang, Y., Tao, Y., Cao, J., Feng, Z."Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity". Oncology Reports 19, no. 2 (2008): 575-581. https://doi.org/10.3892/or.19.2.575
Copy and paste a formatted citation
x
Spandidos Publications style
Qian X, Cheng J, Chen A, Wang Y, Tao Y, Cao J and Feng Z: Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity. Oncol Rep 19: 575-581, 2008.
APA
Qian, X., Cheng, J., Chen, A., Wang, Y., Tao, Y., Cao, J., & Feng, Z. (2008). Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity. Oncology Reports, 19, 575-581. https://doi.org/10.3892/or.19.2.575
MLA
Qian, X., Cheng, J., Chen, A., Wang, Y., Tao, Y., Cao, J., Feng, Z."Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity". Oncology Reports 19.2 (2008): 575-581.
Chicago
Qian, X., Cheng, J., Chen, A., Wang, Y., Tao, Y., Cao, J., Feng, Z."Long-term effects of short hairpin RNA-targeted human telomerase reverse transcriptase on suppression of SGC-7901 cell proliferation by inhibition of telomerase activity". Oncology Reports 19, no. 2 (2008): 575-581. https://doi.org/10.3892/or.19.2.575
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