PDGFRα/β expression correlates with the metastatic behavior of human colorectal cancer: A possible rationale for a molecular targeting strategy

  • Authors:
    • Thomas C. Wehler
    • Kirsten Frerichs
    • Claudine Graf
    • Daniel Drescher
    • Katrin Schimanski
    • Stefan Biesterfeld
    • Martin R. Berger
    • Stephan Kanzler
    • Theodor Junginger
    • Peter R. Galle
    • Markus Moehler
    • Ines Gockel
    • Carl C. Schimanski
  • View Affiliations

  • Published online on: March 1, 2008     https://doi.org/10.3892/or.19.3.697
  • Pages: 697-704
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Abstract

As new multi-target tyrosine kinase inhibitors are emerging in the therapy of various malignancies, our aim was to define the co-expression pattern of receptor-tyrosine-kinase platelet-derived growth factor receptors α and β (PDGFRα/β) in human colorectal cancer. The co-expression pattern of PDGFRα/β was analyzed by RT-PCR in 99 histologically confirmed human colorectal carcinomas and five colorectal cancer cell lines. In addition, immunohistochemical (IHC) staining was applied for confirmation of expression and analysis of receptor tyrosine kinase (RTK) localisation. The colorectal cancer cell lines that were analysed revealed varying expression intensities of PDGFRα and PDGFRβ. The majority of human colorectal cancer specimens revealed a PDGFRα (83%) or PDGFRβ (60%) expression. While PDGFRα showed a predominantly cytoplasmic staining in tumor cells as well as in stromal pericytes, PDGFRβ was restricted to stromal pericytes only. Furthermore, PDGFRα expression significantly correlated with lymph node metastasis (P=0.0082) and advanced UICC stages III/IV (P=0.018) in older patients (P=0.043). PDGFRβ expression only revealed a trend towards lymphatic dissemination (P=0.099). Co-expression of PDGFRα/β occurred in 57% of the colorectal cancer samples, whereas another 29% of the samples depicted mono-expression of PDGFRα or PDGFRβ. Notably, PDGFRα/β expression significantly correlated with lymphatic metastasis (P=0.007) and advanced UICC stages III/IV (P=0.017) in older patients (P=0.03). In summary, our results revealed that PDGFRα/β expression significantly correlates with lymphatic dissemination and therefore encourages application of PDGFRα/β RTK-inhibitors within a combination therapy.

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March 2008
Volume 19 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wehler TC, Frerichs K, Graf C, Drescher D, Schimanski K, Biesterfeld S, Berger MR, Kanzler S, Junginger T, Galle PR, Galle PR, et al: PDGFRα/β expression correlates with the metastatic behavior of human colorectal cancer: A possible rationale for a molecular targeting strategy. Oncol Rep 19: 697-704, 2008
APA
Wehler, T.C., Frerichs, K., Graf, C., Drescher, D., Schimanski, K., Biesterfeld, S. ... Schimanski, C.C. (2008). PDGFRα/β expression correlates with the metastatic behavior of human colorectal cancer: A possible rationale for a molecular targeting strategy. Oncology Reports, 19, 697-704. https://doi.org/10.3892/or.19.3.697
MLA
Wehler, T. C., Frerichs, K., Graf, C., Drescher, D., Schimanski, K., Biesterfeld, S., Berger, M. R., Kanzler, S., Junginger, T., Galle, P. R., Moehler, M., Gockel, I., Schimanski, C. C."PDGFRα/β expression correlates with the metastatic behavior of human colorectal cancer: A possible rationale for a molecular targeting strategy". Oncology Reports 19.3 (2008): 697-704.
Chicago
Wehler, T. C., Frerichs, K., Graf, C., Drescher, D., Schimanski, K., Biesterfeld, S., Berger, M. R., Kanzler, S., Junginger, T., Galle, P. R., Moehler, M., Gockel, I., Schimanski, C. C."PDGFRα/β expression correlates with the metastatic behavior of human colorectal cancer: A possible rationale for a molecular targeting strategy". Oncology Reports 19, no. 3 (2008): 697-704. https://doi.org/10.3892/or.19.3.697