Overexpression of humbug promotes malignant progression in human gastric cancer cells

  • Authors:
    • Jeong-Hyung Lee
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  • Published online on: March 1, 2008     https://doi.org/10.3892/or.19.3.795
  • Pages: 795-800
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Abstract

Two human gastric cancer cell lines of differing invasive potential, SNU-484 and SNU-638 cells, were examined using subtractive suppression hybridization in a search for any genes associated with metastasis. Of the eight cDNAs identified as being differentially expressed genes, it was determined that humbug, which encodes a truncated isoform of aspartyl (asparaginyl) β-hydroxylase (AAH) missing catalytic domain, was overexpressed in highly invasive SNU-638 cells. Expression analysis showed that the mRNA expression level of humbug was correlated with invasive potential in various human gastric cancer cell lines. The forced expression of humbug to the human gastric cancer cell line AGS increased its anchorage-independent growth in 0.3% agar without affecting cell proliferation. Furthermore, humbug-transfected cells migrated more actively and showed an increased invasion rate relative to vector-transfectants or parental AGS in vitro. This is the first demonstration that humbug, a truncated form of AAH, can be overexpressed during the malignant progression of human gastric cancer cells and that it can function as a metastasis-inducing gene.

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March 2008
Volume 19 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lee J: Overexpression of humbug promotes malignant progression in human gastric cancer cells. Oncol Rep 19: 795-800, 2008
APA
Lee, J. (2008). Overexpression of humbug promotes malignant progression in human gastric cancer cells. Oncology Reports, 19, 795-800. https://doi.org/10.3892/or.19.3.795
MLA
Lee, J."Overexpression of humbug promotes malignant progression in human gastric cancer cells". Oncology Reports 19.3 (2008): 795-800.
Chicago
Lee, J."Overexpression of humbug promotes malignant progression in human gastric cancer cells". Oncology Reports 19, no. 3 (2008): 795-800. https://doi.org/10.3892/or.19.3.795