Clodronate inhibits angiogenesis in vitro and in vivo

  • Authors:
    • D. Ribatti
    • N. Maruotti
    • B. Nico
    • V. Longo
    • D. Mangieri
    • A. Vacca
    • F. P. Cantatore
  • View Affiliations

  • Published online on: May 1, 2008     https://doi.org/10.3892/or.19.5.1109
  • Pages: 1109-1112
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Abstract

The effects of amino-bisphosphonate clodronate on endothelial cell functions involved in angiogenesis, namely proliferation and morphogenesis on matrigel were tested in vitro, whereas its effects on angiogenesis were studied in vivo. This was performed by using the chick embryo chorioallantoic membrane (CAM) assay. In vitro, clodronate inhibited the endothelial cell proliferation in a dose-dependent fashion, peaking at 30 µM. At the same concentration, clodronate inhibited the fibroblast growth factor-2 (FGF-2)-induced capillary-like tube formation in the morphogenesis assay on matrigel. In vivo, when tested with the CAM assay, clodronate again displayed the capability to inhibit FGF-2-induced angiogenesis. Overall, these results suggest that antiangiogenesis by clodronate can be used to treat a wide spectrum of angiogenesis-dependent diseases, including certain chronic inflammatory diseases and cancer.

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May 2008
Volume 19 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ribatti D, Maruotti N, Nico B, Longo V, Mangieri D, Vacca A and Cantatore FP: Clodronate inhibits angiogenesis in vitro and in vivo. Oncol Rep 19: 1109-1112, 2008
APA
Ribatti, D., Maruotti, N., Nico, B., Longo, V., Mangieri, D., Vacca, A., & Cantatore, F.P. (2008). Clodronate inhibits angiogenesis in vitro and in vivo. Oncology Reports, 19, 1109-1112. https://doi.org/10.3892/or.19.5.1109
MLA
Ribatti, D., Maruotti, N., Nico, B., Longo, V., Mangieri, D., Vacca, A., Cantatore, F. P."Clodronate inhibits angiogenesis in vitro and in vivo". Oncology Reports 19.5 (2008): 1109-1112.
Chicago
Ribatti, D., Maruotti, N., Nico, B., Longo, V., Mangieri, D., Vacca, A., Cantatore, F. P."Clodronate inhibits angiogenesis in vitro and in vivo". Oncology Reports 19, no. 5 (2008): 1109-1112. https://doi.org/10.3892/or.19.5.1109