Efficacy of oncolytic reovirus against human breast cancer cells

  • Authors:
    • Yoko Hata
    • Tsuyoshi Etoh
    • Masafumi Inomata
    • Norio Shiraishi
    • Akira Nishizono
    • Seigo Kitano
  • View Affiliations

  • Published online on: June 1, 2008     https://doi.org/10.3892/or.19.6.1395
  • Pages: 1395-1398
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Abstract

Human reovirus can replicate and induce tumor cell lysis in several cancer types with an activated Ras signaling pathway. The aim of this study was to investigate the oncolytic effect of reovirus against breast cancer cells, and clarify the relationship between the susceptibility to reovirus and HER2 expression, which is associated with the Ras signaling pathway. Reovirus (serotype 3), 6 human breast cancer cell lines and normal mammary gland epithelial cell line were used in this study. The mRNA expression of HER2 receptor was examined by RT-PCR, and the protein of HER2 and activated Ras protein were examined by a Western blot analysis. In vitro, the cytopathic effects, viral protein synthesis, and cell viability on infection by reovirus were examined. Reovirus can infect all the 6 examined cancer cell lines, but the control cell line was not susceptible to the reovirus infection. The cytopathic effect appeared from day 1 after infection, and a 50% or greater cytotolysis was demonstrated at day 7 after infection. The Ras activities in all examined cell lines were higher than those in the control cell line. No relationship was observed in the susceptibility to the reovirus and HER2 expression. The increased Ras activity itself, regardless of the HER2 expression, may therefore play an important role in the susceptibility to reovirus of breast cancer cells. As a result, breast cancer may thus become a candidate target for oncolytic reovirus therapy.

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June 2008
Volume 19 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Hata Y, Etoh T, Inomata M, Shiraishi N, Nishizono A and Kitano S: Efficacy of oncolytic reovirus against human breast cancer cells. Oncol Rep 19: 1395-1398, 2008
APA
Hata, Y., Etoh, T., Inomata, M., Shiraishi, N., Nishizono, A., & Kitano, S. (2008). Efficacy of oncolytic reovirus against human breast cancer cells. Oncology Reports, 19, 1395-1398. https://doi.org/10.3892/or.19.6.1395
MLA
Hata, Y., Etoh, T., Inomata, M., Shiraishi, N., Nishizono, A., Kitano, S."Efficacy of oncolytic reovirus against human breast cancer cells". Oncology Reports 19.6 (2008): 1395-1398.
Chicago
Hata, Y., Etoh, T., Inomata, M., Shiraishi, N., Nishizono, A., Kitano, S."Efficacy of oncolytic reovirus against human breast cancer cells". Oncology Reports 19, no. 6 (2008): 1395-1398. https://doi.org/10.3892/or.19.6.1395