Tissue factor expression pattern in human non-small cell lung cancer tissues indicate increased blood thrombogenicity and tumor metastasis

  • Authors:
    • Petra Goldin-Lang
    • Quoc-Viet Tran
    • Iduna Fichtner
    • Andreas Eisenreich
    • Silvio Antoniak
    • Karsten Schulze
    • Sarah E. Coupland
    • Wolfgang Poller
    • Heinz-Peter Schultheiss
    • Ursula Rauch
  • View Affiliations

  • Published online on: July 1, 2008     https://doi.org/10.3892/or.20.1.123
  • Pages: 123-128
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Abstract

Non-small cell lung cancer (NSCLC) comprises of 75% of all lung cancers. Human full length tissue factor (flHTF), the physiological initiator of blood coagulation, is aberrantly expressed in certain solid tumors. FlHTF and its soluble isoform, alternatively spliced human tissue factor (asHTF), have been shown to contribute to thrombogenicity of the blood of healthy individuals. The aim of this study was to quantify flHTF and asHTF on mRNA and protein levels (using immunohistochemistry, immunoblotting, and ELISA) on a panel of human NSCLC tissue and plasma specimens. The tissue factor (TF) expression of 21 pulmonary adenomatous (AC) and 12 normal healthy tissues was assessed by real-time qRT-PCR. The TF protein concentration was quantified by ELISA in a subset of 11 AC and 9 normal tissue specimens as well as in the plasma of 13 lung cancer patients and 15 healthy controls. We found a significant increase in the ratio of flHTF/HGAPDH mRNA in AC (0.24±0.06 vs. 0.07±0.01; p=0.02 vs. controls) and in asHTF/HGAPDH mRNA (0.027±0.01 vs. 0.004±0.001; p=0.03 AC vs. controls). AsHTF mRNA expression was significantly lower in patients with stage IA disease compared to patients with higher grade stages, pointing to TF as being a marker of malignancy and metastases. TF protein of lung tumors was significantly increased in AC (p=0.004 vs. controls). TF in plasma was up-regulated in lung cancer patients (334.9±95.4 vs. 124.1±14.8 pg/ml; p=0.02 vs. controls). Immunohistochemical and immunoblotting data are in line with the increased TF expression, showing elevated blood thrombogenicity of NSCLC patients. The up-regulation of flHTF and, especially, asHTF in AC suggests not only a raised risk of thrombosis, but also of tumor progression, thereby, indicating a poor prognosis in these patients.

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July 2008
Volume 20 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Goldin-Lang P, Tran Q, Fichtner I, Eisenreich A, Antoniak S, Schulze K, Coupland SE, Poller W, Schultheiss H, Rauch U, Rauch U, et al: Tissue factor expression pattern in human non-small cell lung cancer tissues indicate increased blood thrombogenicity and tumor metastasis. Oncol Rep 20: 123-128, 2008
APA
Goldin-Lang, P., Tran, Q., Fichtner, I., Eisenreich, A., Antoniak, S., Schulze, K. ... Rauch, U. (2008). Tissue factor expression pattern in human non-small cell lung cancer tissues indicate increased blood thrombogenicity and tumor metastasis. Oncology Reports, 20, 123-128. https://doi.org/10.3892/or.20.1.123
MLA
Goldin-Lang, P., Tran, Q., Fichtner, I., Eisenreich, A., Antoniak, S., Schulze, K., Coupland, S. E., Poller, W., Schultheiss, H., Rauch, U."Tissue factor expression pattern in human non-small cell lung cancer tissues indicate increased blood thrombogenicity and tumor metastasis". Oncology Reports 20.1 (2008): 123-128.
Chicago
Goldin-Lang, P., Tran, Q., Fichtner, I., Eisenreich, A., Antoniak, S., Schulze, K., Coupland, S. E., Poller, W., Schultheiss, H., Rauch, U."Tissue factor expression pattern in human non-small cell lung cancer tissues indicate increased blood thrombogenicity and tumor metastasis". Oncology Reports 20, no. 1 (2008): 123-128. https://doi.org/10.3892/or.20.1.123