Knockdown of the Bmi-1 oncogene inhibits cell proliferation and induces cell apoptosis and is involved in the decrease of Akt phosphorylation in the human breast carcinoma cell line MCF-7

  • Authors:
    • Zhengshun Xu
    • Hongtao Liu
    • Xinquan Lv
    • Yuqiong Liu
    • Shenglei Li
    • Huixiang Li
  • View Affiliations

  • Published online on: December 7, 2010     https://doi.org/10.3892/or.2010.1078
  • Pages: 409-418
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Abstract

It is well documented that B cell-specific Moloney murine leukemia virus integration site 1 (Bmi-1), widely overexpressed in the vast majority of malignancies, plays an essential role in the occurrence and development of several different tumors. Here, we report Bmi-1 siRNA-mediated cell proliferation inhibition and cell apoptosis in vitro and in vivo in the human breast carcinoma cell line MCF-7. Our results demonstrated that Bmi-1 siRNA effectively down-regulated the expression of Bmi-1, inhibited cell proliferation in vitro and in vivo, evoked cell cycle arrest in the G0/G1 phase and induced cell apoptosis in MCF-7 cells, coupled with decrease in cyclin D1, cyclin E, cdk2, bcl-2 and Ki-67 expression and Akt phosphorylation levels and an increase of p21 and bax expression and activities of caspase-3/-9. Taken together, our results suggest that Bmi-1 may be a potential molecular target for the therapy of breast carcinoma.

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February 2011
Volume 25 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xu Z, Liu H, Lv X, Liu Y, Li S and Li H: Knockdown of the Bmi-1 oncogene inhibits cell proliferation and induces cell apoptosis and is involved in the decrease of Akt phosphorylation in the human breast carcinoma cell line MCF-7. Oncol Rep 25: 409-418, 2011.
APA
Xu, Z., Liu, H., Lv, X., Liu, Y., Li, S., & Li, H. (2011). Knockdown of the Bmi-1 oncogene inhibits cell proliferation and induces cell apoptosis and is involved in the decrease of Akt phosphorylation in the human breast carcinoma cell line MCF-7. Oncology Reports, 25, 409-418. https://doi.org/10.3892/or.2010.1078
MLA
Xu, Z., Liu, H., Lv, X., Liu, Y., Li, S., Li, H."Knockdown of the Bmi-1 oncogene inhibits cell proliferation and induces cell apoptosis and is involved in the decrease of Akt phosphorylation in the human breast carcinoma cell line MCF-7". Oncology Reports 25.2 (2011): 409-418.
Chicago
Xu, Z., Liu, H., Lv, X., Liu, Y., Li, S., Li, H."Knockdown of the Bmi-1 oncogene inhibits cell proliferation and induces cell apoptosis and is involved in the decrease of Akt phosphorylation in the human breast carcinoma cell line MCF-7". Oncology Reports 25, no. 2 (2011): 409-418. https://doi.org/10.3892/or.2010.1078