Acute application of cisplatin affects methylation status in neuroblastoma cells

  • Authors:
    • Keiichi Tabata
    • Hayato Sakai
    • Ryosuke Nakajima
    • Reiko Saya-Nishimura
    • Kou Motani
    • Soichiro Okano
    • Yasuko Shibata
    • Yoshimitsu Abiko
    • Takashi Suzuki
  • View Affiliations

  • Published online on: March 18, 2011     https://doi.org/10.3892/or.2011.1222
  • Pages: 1655-1660
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Abstract

The pharmacological mechanism of the anti-cancer effect of cisplatin is well known to be DNA intercalation, but the direct or indirect effects of cisplatin on protein expression in cancer cells remain to be explained. In this study, we used a proteomic approach to clarify the early impact of cisplatin on protein expression. In a 2-dimensional gel electrophoresis proteomic experiment, the application of cisplatin for 24 h increased the expression of four proteins and decreased the levels of one protein in neuroblastoma IMR-32 cells. Levels of S-adenosyl-L-homocysteine hydrolase, a key enzyme in methylation metabolism, were increased the most. Therefore, we examined the methylation status of histone proteins. Histone H3K9 methylation was reduced by the application of cisplatin for 24 h. These results suggest that acute cisplatin treatment alters methylation status. Thus, these data can help clarify the unknown pharmacological mechanisms of cisplatin, including the anticancer effect, adverse effects and/or the mechanism of drug resistance.

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June 2011
Volume 25 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Tabata, K., Sakai, H., Nakajima, R., Saya-Nishimura, R., Motani, K., Okano, S. ... Suzuki, T. (2011). Acute application of cisplatin affects methylation status in neuroblastoma cells. Oncology Reports, 25, 1655-1660. https://doi.org/10.3892/or.2011.1222
MLA
Tabata, K., Sakai, H., Nakajima, R., Saya-Nishimura, R., Motani, K., Okano, S., Shibata, Y., Abiko, Y., Suzuki, T."Acute application of cisplatin affects methylation status in neuroblastoma cells". Oncology Reports 25.6 (2011): 1655-1660.
Chicago
Tabata, K., Sakai, H., Nakajima, R., Saya-Nishimura, R., Motani, K., Okano, S., Shibata, Y., Abiko, Y., Suzuki, T."Acute application of cisplatin affects methylation status in neuroblastoma cells". Oncology Reports 25, no. 6 (2011): 1655-1660. https://doi.org/10.3892/or.2011.1222