The histone deacetylase inhibitor, Trichostatin A, induces G2/M phase arrest and apoptosis in YD-10B oral squamous carcinoma cells

  • Authors:
    • Trinh Duc Anh
    • Mee-Young Ahn
    • Soo-A Kim
    • Jung-Hoon Yoon
    • Sang-Gun Ahn
  • View Affiliations

  • Published online on: October 6, 2011     https://doi.org/10.3892/or.2011.1496
  • Pages: 455-460
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Abstract

Histone acetylation is one of the key chromatin modifications that control gene transcription during development and tumorigenesis. Recently, it was reported that the histone deacetylase inhibitor, Trichostatin A (TSA), induces growth arrest and apoptosis in tumors. However, the molecular mechanisms responsible for its antitumor effects are not clear. The purpose of this study was to investigate the effect of TSA on human oral squamous carcinoma cells and to determine the mechanisms underlying the antitumor activity of TSA. MTT assays showed that TSA inhibited cell proliferation in YD-10B cells. TSA also effectively arrested cell cycle progression at the G2/M phase through the up-regulation of p21waf expression, down-regulation of Cyclin B1 and reduction of the inhibitory phophorylation of Cdc2. In addition, mitochondrial membrane destruction was induced by a 48 h TSA treatment. TSA also induced cytochrome c release and proteolytic activation of caspase 3 and caspase 7 in YD-10B cells. Taken together, these observations in YD-10B oral cancer cells reveal the potential value of TSA in inhibiting oral tumor growth.

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February 2012
Volume 27 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Anh TD, Ahn M, Kim S, Yoon J and Ahn S: The histone deacetylase inhibitor, Trichostatin A, induces G2/M phase arrest and apoptosis in YD-10B oral squamous carcinoma cells. Oncol Rep 27: 455-460, 2012
APA
Anh, T.D., Ahn, M., Kim, S., Yoon, J., & Ahn, S. (2012). The histone deacetylase inhibitor, Trichostatin A, induces G2/M phase arrest and apoptosis in YD-10B oral squamous carcinoma cells. Oncology Reports, 27, 455-460. https://doi.org/10.3892/or.2011.1496
MLA
Anh, T. D., Ahn, M., Kim, S., Yoon, J., Ahn, S."The histone deacetylase inhibitor, Trichostatin A, induces G2/M phase arrest and apoptosis in YD-10B oral squamous carcinoma cells". Oncology Reports 27.2 (2012): 455-460.
Chicago
Anh, T. D., Ahn, M., Kim, S., Yoon, J., Ahn, S."The histone deacetylase inhibitor, Trichostatin A, induces G2/M phase arrest and apoptosis in YD-10B oral squamous carcinoma cells". Oncology Reports 27, no. 2 (2012): 455-460. https://doi.org/10.3892/or.2011.1496