A novel TFF2 splice variant (∆EX2TFF2) correlates with longer overall survival time in cholangiocarcinoma

  • Authors:
    • Surasee Kamlua
    • Siriporn Patrakitkomjorn
    • Patcharee Jearanaikoon
    • Trevelyan R. Menheniott
    • Andrew S. Giraud
    • Temduang Limpaiboon
  • View Affiliations

  • Published online on: December 7, 2011     https://doi.org/10.3892/or.2011.1583
  • Pages: 1207-1212
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Abstract

Trefoil factor 2 (TFF2) is a member of trefoil factor family found to be overexpressed in many cancers including cholangiocarcinoma (CCA). The majority of studies have focused on wild-type TFF2 (wtTFF2) expression, but information regarding alternative splicing variants of TFF2 mRNA has not been reported. In this study, we aimed to identify and quantify a novel TFF2 splice variant in cholangiocarcinoma (CCA). Seventy-eight tumors and 15 normal adjacent tissues were quantified for the expression of the TFF2 splice variant relative to wild-type (wt) TFF2 mRNA using quantitative reverse transcriptase polymerase chain reaction (QRT-PCR). The ratio of TFF2 splice variant against wtTFF2 was analyzed for associations with clinical parameters. We found a novel TFF2 splice variant, exon 2 skipping (∆EX2TFF2), resulting in a stop codon (TAG) at exon 1. The ∆EX2TFF2/wtTFF2 ratio in tumors was significantly higher than in normal tissue (P<0.01). Interestingly, high ∆EX2TFF2/wtTFF2 ratio was significantly associated with good prognosis compared with low ratio (P=0.017). In contrast, the presence of wtTFF2 protein was associated with poor survival of CCA patients (P=0.034). This is the first report of a trefoil factor splice variant and its potential application as a prognostic biomarker in CCA.
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April 2012
Volume 27 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kamlua S, Patrakitkomjorn S, Jearanaikoon P, Menheniott TR, Giraud AS and Limpaiboon T: A novel TFF2 splice variant (∆EX2TFF2) correlates with longer overall survival time in cholangiocarcinoma. Oncol Rep 27: 1207-1212, 2012
APA
Kamlua, S., Patrakitkomjorn, S., Jearanaikoon, P., Menheniott, T.R., Giraud, A.S., & Limpaiboon, T. (2012). A novel TFF2 splice variant (∆EX2TFF2) correlates with longer overall survival time in cholangiocarcinoma. Oncology Reports, 27, 1207-1212. https://doi.org/10.3892/or.2011.1583
MLA
Kamlua, S., Patrakitkomjorn, S., Jearanaikoon, P., Menheniott, T. R., Giraud, A. S., Limpaiboon, T."A novel TFF2 splice variant (∆EX2TFF2) correlates with longer overall survival time in cholangiocarcinoma". Oncology Reports 27.4 (2012): 1207-1212.
Chicago
Kamlua, S., Patrakitkomjorn, S., Jearanaikoon, P., Menheniott, T. R., Giraud, A. S., Limpaiboon, T."A novel TFF2 splice variant (∆EX2TFF2) correlates with longer overall survival time in cholangiocarcinoma". Oncology Reports 27, no. 4 (2012): 1207-1212. https://doi.org/10.3892/or.2011.1583