A novel TFF2 splice variant (∆EX2TFF2) correlates with longer overall survival time in cholangiocarcinoma

Kamlua,Surasee; Patrakitkomjorn,Siriporn; Jearanaikoon,Patcharee; Menheniott,Trevelyan  R.; Giraud,Andrew  S.; Limpaiboon,Temduang

doi:10.3892/or.2011.1583

April 2012 Volume 27 Issue 4

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April 2012 Volume 27 Issue 4

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Article

A novel TFF2 splice variant (∆EX2TFF2) correlates with longer overall survival time in cholangiocarcinoma

Authors:
- Surasee Kamlua
- Siriporn Patrakitkomjorn
- Patcharee Jearanaikoon
- Trevelyan R. Menheniott
- Andrew S. Giraud
- Temduang Limpaiboon
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Affiliations: Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
Pages: 1207-1212
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Published online on: December 7, 2011

https://doi.org/10.3892/or.2011.1583
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Abstract

Trefoil factor 2 (TFF2) is a member of trefoil factor family found to be overexpressed in many cancers including cholangiocarcinoma (CCA). The majority of studies have focused on wild-type TFF2 (wtTFF2) expression, but information regarding alternative splicing variants of TFF2 mRNA has not been reported. In this study, we aimed to identify and quantify a novel TFF2 splice variant in cholangiocarcinoma (CCA). Seventy-eight tumors and 15 normal adjacent tissues were quantified for the expression of the TFF2 splice variant relative to wild-type (wt) TFF2 mRNA using quantitative reverse transcriptase polymerase chain reaction (QRT-PCR). The ratio of TFF2 splice variant against wtTFF2 was analyzed for associations with clinical parameters. We found a novel TFF2 splice variant, exon 2 skipping (∆EX2TFF2), resulting in a stop codon (TAG) at exon 1. The ∆EX2TFF2/wtTFF2 ratio in tumors was significantly higher than in normal tissue (P<0.01). Interestingly, high ∆EX2TFF2/wtTFF2 ratio was significantly associated with good prognosis compared with low ratio (P=0.017). In contrast, the presence of wtTFF2 protein was associated with poor survival of CCA patients (P=0.034). This is the first report of a trefoil factor splice variant and its potential application as a prognostic biomarker in CCA.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A novel TFF2 splice variant (∆EX2TFF2) correlates with longer overall survival time in cholangiocarcinoma

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