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Article

Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells

  • Authors:
    • Hyunsoo Shin
    • Yeo Song Lee
    • Yong Chan Lee
  • View Affiliations / Copyright

    Affiliations: Department of Internal Medicine, Institute of Gastroenterology, Yonsei University College of Medicine, Seoul, Republic of Korea, Department of Internal Medicine, Yonsei University College of Medicine, 134 Shinchon-Dong, Seodaemun-Ku, Seoul 120-752, Republic of Korea
  • Pages: 1111-1115
    |
    Published online on: December 8, 2011
       https://doi.org/10.3892/or.2011.1585
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Abstract

Epigenetic mechanisms of histone acetylation/deacetylation play an important role in the regulation of gene expression associated with the cell cycle and apoptosis. Recently, sodium butyrate, a histone deacetylase (HDAC) inhibitor, has been shown to exhibit anticancer effects via differentiation and apoptosis of cancer cells. Sodium butyrate may be a potential anticancer chemotherapeutic drug; however, the precise mechanism underlying the anticancer effects of sodium butyrate has not been clearly elucidated. In the present study, we investigated the role of death-associated protein kinase (DAPK) on the apoptosis of human gastric cancer cells induced by sodium butyrate. We observed that sodium butyrate induced apoptosis in human gastric cancer cells. Treatment with the HDAC inhibitor sodium butyrate increased the expression of caspase-3 and DAPK1/2 genes but decreased the expression of Bcl-2 in human gastric cancer cells. The expression of DAPK3, p53 and p21 were not altered by sodium butyrate treatment. Analysis of the general expression patterns revealed that sodium butyrate increased the expression of DAPK1/2 but decreased the expression of FAK and induced changes in the proliferation of apoptosis-related genes in human gastric cancer cells. These data suggest that DAPK expression prompts apoptosis by reducing the FAK protein level in sodium butyrate-induced apoptosis of human gastric cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Shin H, Lee YS and Lee YC: Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells. Oncol Rep 27: 1111-1115, 2012.
APA
Shin, H., Lee, Y.S., & Lee, Y.C. (2012). Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells. Oncology Reports, 27, 1111-1115. https://doi.org/10.3892/or.2011.1585
MLA
Shin, H., Lee, Y. S., Lee, Y. C."Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells". Oncology Reports 27.4 (2012): 1111-1115.
Chicago
Shin, H., Lee, Y. S., Lee, Y. C."Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells". Oncology Reports 27, no. 4 (2012): 1111-1115. https://doi.org/10.3892/or.2011.1585
Copy and paste a formatted citation
x
Spandidos Publications style
Shin H, Lee YS and Lee YC: Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells. Oncol Rep 27: 1111-1115, 2012.
APA
Shin, H., Lee, Y.S., & Lee, Y.C. (2012). Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells. Oncology Reports, 27, 1111-1115. https://doi.org/10.3892/or.2011.1585
MLA
Shin, H., Lee, Y. S., Lee, Y. C."Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells". Oncology Reports 27.4 (2012): 1111-1115.
Chicago
Shin, H., Lee, Y. S., Lee, Y. C."Sodium butyrate-induced DAPK-mediated apoptosis in human gastric cancer cells". Oncology Reports 27, no. 4 (2012): 1111-1115. https://doi.org/10.3892/or.2011.1585
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