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April 2012 Volume 27 Issue 4

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Article

The effects of cyclooxygenase-2 gene silencing by siRNA on cell proliferation, cell apoptosis, cell cycle and tumorigenicity of Capan-2 human pancreatic cancer cells

  • Authors:
    • Yingqiang Zhong
    • Zhongsheng Xia
    • Juan Liu
    • Ying Lin
    • Hui Zan
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China, Department of Gastro­enterology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China
  • Pages: 1003-1010
    |
    Published online on: December 19, 2011
       https://doi.org/10.3892/or.2011.1595
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Abstract

The prognosis of pancreatic cancer is still very poor. No specific effective gene therapy for pancreatic cancer has been found. As a key enzyme of the metabolic process of arachidonic acid, cyclooxygenase-2 (COX-2) has been found to be closely related to the tumorigenesis of epithelial cancers. However, the antitumor effect of small interfering RNA (siRNA) targeting COX-2 in pancreatic cancer has not yet been verified. Therefore, the aim of this study was to investigate the effects of COX-2 gene silencing by siRNA on cell proliferation, cell apoptosis, cell cycle and tumorigenicity of pancreatic cancer cells. COX-2 mRNA was detected by RT-PCR and real-time PCR. COX-2 protein was detected by Western blotting. The cell proliferation was measured by cell counting using microscopy. The cell apoptosis and cell cycle were measured by flow cytometry. The tumorigenicity of Capan-2 pancreatic cancer cells transfected with COX-2 siRNA was evaluated using a nude mouse xenograft model. The expression of COX-2 mRNA as well as COX-2 protein were downregulated after COX-2 siRNA transfection. COX-2 siRNA could inhibit the growth of Capan-2 cells significantly by decreasing the cell proliferation, increasing cell apoptosis and regulating cell cycle as well. In vivo experiments demonstrated that the mean volume and weight of subcutaneous xenografts in nude mice derived from Capan-2 cells transfected with COX-2 siRNA were significantly decreased. COX-2 siRNA could inhibit the growth of Capan-2 pancreatic cancer cells and also decrease the tumorigenicity of Capan-2 cells, implicating a new potential therapeutic target in pancreatic cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhong Y, Xia Z, Liu J, Lin Y and Zan H: The effects of cyclooxygenase-2 gene silencing by siRNA on cell proliferation, cell apoptosis, cell cycle and tumorigenicity of Capan-2 human pancreatic cancer cells. Oncol Rep 27: 1003-1010, 2012.
APA
Zhong, Y., Xia, Z., Liu, J., Lin, Y., & Zan, H. (2012). The effects of cyclooxygenase-2 gene silencing by siRNA on cell proliferation, cell apoptosis, cell cycle and tumorigenicity of Capan-2 human pancreatic cancer cells. Oncology Reports, 27, 1003-1010. https://doi.org/10.3892/or.2011.1595
MLA
Zhong, Y., Xia, Z., Liu, J., Lin, Y., Zan, H."The effects of cyclooxygenase-2 gene silencing by siRNA on cell proliferation, cell apoptosis, cell cycle and tumorigenicity of Capan-2 human pancreatic cancer cells". Oncology Reports 27.4 (2012): 1003-1010.
Chicago
Zhong, Y., Xia, Z., Liu, J., Lin, Y., Zan, H."The effects of cyclooxygenase-2 gene silencing by siRNA on cell proliferation, cell apoptosis, cell cycle and tumorigenicity of Capan-2 human pancreatic cancer cells". Oncology Reports 27, no. 4 (2012): 1003-1010. https://doi.org/10.3892/or.2011.1595
Copy and paste a formatted citation
x
Spandidos Publications style
Zhong Y, Xia Z, Liu J, Lin Y and Zan H: The effects of cyclooxygenase-2 gene silencing by siRNA on cell proliferation, cell apoptosis, cell cycle and tumorigenicity of Capan-2 human pancreatic cancer cells. Oncol Rep 27: 1003-1010, 2012.
APA
Zhong, Y., Xia, Z., Liu, J., Lin, Y., & Zan, H. (2012). The effects of cyclooxygenase-2 gene silencing by siRNA on cell proliferation, cell apoptosis, cell cycle and tumorigenicity of Capan-2 human pancreatic cancer cells. Oncology Reports, 27, 1003-1010. https://doi.org/10.3892/or.2011.1595
MLA
Zhong, Y., Xia, Z., Liu, J., Lin, Y., Zan, H."The effects of cyclooxygenase-2 gene silencing by siRNA on cell proliferation, cell apoptosis, cell cycle and tumorigenicity of Capan-2 human pancreatic cancer cells". Oncology Reports 27.4 (2012): 1003-1010.
Chicago
Zhong, Y., Xia, Z., Liu, J., Lin, Y., Zan, H."The effects of cyclooxygenase-2 gene silencing by siRNA on cell proliferation, cell apoptosis, cell cycle and tumorigenicity of Capan-2 human pancreatic cancer cells". Oncology Reports 27, no. 4 (2012): 1003-1010. https://doi.org/10.3892/or.2011.1595
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